PMID- 20719194
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20100819
IS  - 1999-6187 (Electronic)
IS  - 1009-3419 (Linking)
VI  - 12
IP  - 9
DP  - 2009 Sep 20
TI  - [A Novel Method for Detecting p53 Autoantibodies in Sera of Patients with 
      NSCLC.].
PG  - 969-74
LID - 10.3779/j.issn.1009-3419.2009.09.006 [doi]
AB  - BACKGROUND: Serum autoantibody detection is useful means for the early diagnosis 
      and prognosis of cancer. So our objective was to synthesize peptide array to 
      analyse p53 autoantibodies in the sera of patients with non small cell lung 
      cancer (NSCLC). METHODS: Cellulose-bound overlapping peptides (12 mers) derived 
      from p53 wild type protein were synthesized using SOPTs synthesis technique by an 
      AutoSpot robot -ASP SL (Intavis, Germany). The membrane was incubated with 1/400 
      dilutions of p53 monoclonal antibody (Sc-53394) to establish a new approach to 
      detect p53 antibody, and the epitopes of the p53 monoclonal antibody is already 
      known. We analysed the p53 autoantibodies from the sera of NSCLC and controls by 
      peptide array and ELISA. RESULTS: We synthesized on cellulose membranes 
      twelve-amino-acid overlapping peptides which included all of the sequences of the 
      polypeptide chain of p53. The p53 autoantibody was positive in seven cases of 
      thirty patients sera with NSCLC and was negative in sera of the controls, with 
      the same result of ELISA CONCLUSIONS: The peptide array could be applied not only 
      to detect the autoantibodies in the sera of patients with lung cancer, but also 
      to map the epitopes of the autoantibodies which might be useful for the early 
      diagnosis and prognosis of cancer.
FAU - Tang, Kai
AU  - Tang K
AD  - Department of Cellular and Molecular Biology, Beijing TB and Thoracic Tumor 
      Research Institute, Beijing 101149, China.
FAU - Yue, Wentao
AU  - Yue W
FAU - Wang, Yue
AU  - Wang Y
FAU - Zhang, Lina
AU  - Zhang L
FAU - Xu, Shaofa
AU  - Xu S
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Fei Ai Za Zhi
JT  - Zhongguo fei ai za zhi = Chinese journal of lung cancer
JID - 101126433
EDAT- 2010/08/20 06:00
MHDA- 2010/08/20 06:01
CRDT- 2010/08/20 06:00
PHST- 2010/08/20 06:00 [entrez]
PHST- 2010/08/20 06:00 [pubmed]
PHST- 2010/08/20 06:01 [medline]
AID - 10.3779/j.issn.1009-3419.2009.09.006 [doi]
PST - ppublish
SO  - Zhongguo Fei Ai Za Zhi. 2009 Sep 20;12(9):969-74. doi: 
      10.3779/j.issn.1009-3419.2009.09.006.