PMID- 20719236
OWN - NLM
STAT- MEDLINE
DCOM- 20101013
LR  - 20131121
IS  - 1090-2104 (Electronic)
IS  - 0006-291X (Linking)
VI  - 400
IP  - 2
DP  - 2010 Sep 17
TI  - Ephrinb3 induces mesostriatal dopaminergic projection to the striatum.
PG  - 194-9
LID - 10.1016/j.bbrc.2010.08.027 [doi]
AB  - Dopaminergic neurons in midbrain are subdivided into three subsets, forming 
      mesostriatal, mesocortical and mesolimbic projections, respectively. The 
      molecular mechanism specifying mesostriatal projection, which is important for 
      understanding the pathogenesis of Parkinson's disease, remains unclear. To probe 
      the role of axon guide molecule Ephrinb3 in inducing mesostriatal projection, we 
      labeled mesostriatal and mesocortical subset DA neurons with fluorescent 
      microspheres, and purified these subpopulation cells with fluorescence-activated 
      cell sorting (FACS). Moreover, real-time PCR was performed to address the 
      expression of Ephrinb3 in mesostriatal DA neurons, and fluorescence in situ 
      hybridization (FISH) was used to further verify the expression of Ephrinb3 in 
      labeled neurons. The results showed that mesostriatal DA neurons were 
      successfully isolated with retrograde labeling and FACS. Real-time PCR showed 
      that the expression of Ephrinb3 was higher in mesostriatal DA neurons than in 
      mesocortical neurons. Also, Ephrinb3 could be detected in labeled neurons with 
      FISH. Our results indicate that Ephrinb3 is directly involved in the specificity 
      of mesostriatal projection.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Sun, J H
AU  - Sun JH
AD  - Key Laboratory of the Ministry of Education for Experimental Teratology, 
      Department of Anatomy, Shandong University School of Medicine, Jinan 250012, 
      Shandong, PR China. sunjinhao@gmail.com
FAU - Gao, Q
AU  - Gao Q
FAU - Zhang, J
AU  - Zhang J
FAU - Bao, L H
AU  - Bao LH
FAU - Dong, H M
AU  - Dong HM
FAU - Liang, N
AU  - Liang N
FAU - Li, G B
AU  - Li GB
FAU - Li, Z H
AU  - Li ZH
FAU - Gao, Y M
AU  - Gao YM
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100816
PL  - United States
TA  - Biochem Biophys Res Commun
JT  - Biochemical and biophysical research communications
JID - 0372516
RN  - 0 (Ephrin-B3)
RN  - VTD58H1Z2X (Dopamine)
SB  - IM
MH  - Animals
MH  - Cell Separation
MH  - Corpus Striatum/*cytology/metabolism
MH  - Dopamine/*metabolism
MH  - Ephrin-B3/*genetics
MH  - Flow Cytometry
MH  - *Gene Expression
MH  - In Situ Hybridization, Fluorescence
MH  - Mice
MH  - Mice, Inbred Strains
MH  - Microspheres
MH  - Neurons/*metabolism
MH  - Polymerase Chain Reaction
MH  - Staining and Labeling
EDAT- 2010/08/20 06:00
MHDA- 2010/10/14 06:00
CRDT- 2010/08/20 06:00
PHST- 2010/07/30 00:00 [received]
PHST- 2010/08/07 00:00 [accepted]
PHST- 2010/08/20 06:00 [entrez]
PHST- 2010/08/20 06:00 [pubmed]
PHST- 2010/10/14 06:00 [medline]
AID - S0006-291X(10)01513-5 [pii]
AID - 10.1016/j.bbrc.2010.08.027 [doi]
PST - ppublish
SO  - Biochem Biophys Res Commun. 2010 Sep 17;400(2):194-9. doi: 
      10.1016/j.bbrc.2010.08.027. Epub 2010 Aug 16.