PMID- 20719493
OWN - NLM
STAT- MEDLINE
DCOM- 20110331
LR  - 20191210
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 26
IP  - 4
DP  - 2010 Dec 15
TI  - Biosensors coated with sulfated polysaccharides for the detection of hepatocyte 
      growth factor/scatter factor in cell culture medium.
PG  - 1706-9
LID - 10.1016/j.bios.2010.07.065 [doi]
AB  - Process control methods for cell culture bioreactors include on-line monitoring 
      of protein concentrations. Bioreactor samples typically contain high amounts of 
      different proteins. The direct detection of a single protein in this complex 
      medium is a challenging task within the development of biosensors with label-free 
      detection. We introduce the development of a mass-sensitive biosensor based on 
      surface acoustic waves (SAW) for the detection of hepatocyte growth 
      factor/scatter factor (HGF/SF) in the serum containing medium of a miniaturized 
      bioreactor for culturing hepatocytes. The specificity of the biosensor was 
      obtained following two approaches. In the first approach, antibodies against HGF 
      (anti-HGF) were immobilized covalently via an intermediate layer of dicarboxy 
      polyethylene glycol on the biosensor surface. In the second approach, dextran 
      sulfate and fucoidan were used as sensor coatings exploiting the fact that HGF 
      binds specifically to those sulfated polysaccharides. Performing HGF assays, 
      similar results were obtained using biosensors coated with dextran sulfate and 
      biosensors coated with anti-HGF. Even higher sensor signals were obtained using 
      biosensors coated with fucoidan, particularly at 37 degrees C. Therefore, biosensor 
      coatings based on biospecific sulfated polysaccharides offer a simple and 
      cost-saving alternative compared to the commonly used coating with antibodies.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Berger, Manuel
AU  - Berger M
AD  - Karlsruhe Institute of Technology, Institute for Microstructure Technology, 
      Hermann-von-Helmholtz-Platz 1, 76344 Eggenstein-Leopoldshafen, Germany.
FAU - Welle, Alexander
AU  - Welle A
FAU - Gottwald, Eric
AU  - Gottwald E
FAU - Rapp, Michael
AU  - Rapp M
FAU - Lange, Kerstin
AU  - Lange K
LA  - eng
PT  - Comparative Study
PT  - Evaluation Study
PT  - Journal Article
DEP - 20100723
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (Antibodies, Immobilized)
RN  - 0 (Coated Materials, Biocompatible)
RN  - 0 (Culture Media)
RN  - 0 (HGF protein, human)
RN  - 0 (Polysaccharides)
RN  - 67256-21-7 (Hepatocyte Growth Factor)
RN  - 9042-14-2 (Dextran Sulfate)
RN  - 9072-19-9 (fucoidan)
SB  - IM
MH  - Acoustics
MH  - Antibodies, Immobilized
MH  - Bioreactors
MH  - Biosensing Techniques/*methods
MH  - Coated Materials, Biocompatible
MH  - Culture Media/*analysis
MH  - Dextran Sulfate
MH  - Hep G2 Cells
MH  - Hepatocyte Growth Factor/*analysis/immunology
MH  - Humans
MH  - *Polysaccharides
EDAT- 2010/08/20 06:00
MHDA- 2011/04/01 06:00
CRDT- 2010/08/20 06:00
PHST- 2010/04/12 00:00 [received]
PHST- 2010/07/02 00:00 [revised]
PHST- 2010/07/16 00:00 [accepted]
PHST- 2010/08/20 06:00 [entrez]
PHST- 2010/08/20 06:00 [pubmed]
PHST- 2011/04/01 06:00 [medline]
AID - S0956-5663(10)00440-9 [pii]
AID - 10.1016/j.bios.2010.07.065 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2010 Dec 15;26(4):1706-9. doi: 10.1016/j.bios.2010.07.065. 
      Epub 2010 Jul 23.