PMID- 20721247
OWN - NLM
STAT- MEDLINE
DCOM- 20110919
LR  - 20220331
IS  - 1671-167X (Print)
IS  - 1671-167X (Linking)
VI  - 42
IP  - 4
DP  - 2010 Aug 18
TI  - [Clinical utility of fluorescence in situ hybridization improves the sensitivity 
      in the diagnosis of upper urinary tract urothelial carcinoma].
PG  - 381-5
AB  - OBJECTIVE: To assess the clinical utility of a fluorescence in situ hybridization 
      (FISH) assay as a non-invasive method for diagnosing and monitoring urothelial 
      carcinoma (UC) in the upper urinary tract (UUT). METHODS: Urine specimens from 63 
      consecutive patients with UUT-UC and 69 controls with benign disease were 
      analyzed by means of cytology and FISH. For FISH analysis, labeled probes 
      specific for chromosomes 3, 7, and 17 and for the p16 (9p21) gene were used to 
      assess chromosomal abnormalities indicative of malignancy.Sensitivity and 
      specificity of both techniques were determined and compared. The frequency of 
      chromosomal aberrations of malignant cells from UUT-UC was also determined. 
      RESULTS: Of 63 patients with UUT-UC, FISH affords an overall sensitivity of 84.1% 
      (53/63), the figure being 71.4% (20/28) for PTa and PT1 tumors,94.3% (33/35) for 
      PT2-4 tumors. The sensitivities of urine cytology were 35.7% (10/28) for PTa and 
      PT1 tumor,45.7% (16/35)for PT2-4 tumors,with an overall sensitivity of 41.3% 
      (26/63). The sensitivities of the two methods for the low grade tumors were 80% 
      (20/25) and 44% (11/25), and for high grade tumors were 86.8% (33/38) and 39.5% 
      (15/38), respectively. Specificities for FISH and urine cytology were 91.3% 
      (63/69) and 94.2% (65/69)respectively. CONCLUSION: According to the results,the 
      sensitivity of FISH for the detection of UUT-UC is superior to that of urine 
      cytology and the specificities of FISH and urine cytology are not significantly 
      different. FISH can promote the diagnosis of UUT-UC, especially for the low stage 
      and low grade cases,it may be a new promising non-invasive method for the 
      diagnosis of UUT-UC.
FAU - Mu, Da-wei
AU  - Mu DW
AD  - Department of Urology, Peking University First Hospital, Institute of Urology, 
      Peking University, Beijing 100034, China.
FAU - Zhou, Li-qun
AU  - Zhou LQ
FAU - Ding, Yi
AU  - Ding Y
FAU - He, Zhi-song
AU  - He ZS
FAU - Wang, Ying
AU  - Wang Y
FAU - He, Qun
AU  - He Q
FAU - Yang, Xin-yu
AU  - Yang XY
LA  - chi
PT  - Comparative Study
PT  - English Abstract
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - China
TA  - Beijing Da Xue Xue Bao Yi Xue Ban
JT  - Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health 
      sciences
JID - 101125284
SB  - IM
MH  - Adult
MH  - Aged
MH  - Aged, 80 and over
MH  - Carcinoma/*diagnosis/pathology
MH  - Female
MH  - Humans
MH  - *In Situ Hybridization, Fluorescence
MH  - Kidney Neoplasms/*diagnosis/pathology
MH  - *Kidney Pelvis
MH  - Male
MH  - Middle Aged
MH  - Sensitivity and Specificity
MH  - Ureteral Neoplasms/*diagnosis/pathology
MH  - Urothelium/pathology
EDAT- 2010/08/20 06:00
MHDA- 2011/09/20 06:00
CRDT- 2010/08/20 06:00
PHST- 2010/08/20 06:00 [entrez]
PHST- 2010/08/20 06:00 [pubmed]
PHST- 2011/09/20 06:00 [medline]
PST - ppublish
SO  - Beijing Da Xue Xue Bao Yi Xue Ban. 2010 Aug 18;42(4):381-5.