PMID- 20722875
OWN - NLM
STAT- MEDLINE
DCOM- 20110321
LR  - 20181201
IS  - 1365-2672 (Electronic)
IS  - 1364-5072 (Linking)
VI  - 109
IP  - 6
DP  - 2010 Dec
TI  - Influence of the maize silage to grass silage ratio and feed particle size of 
      rations for ruminants on the community structure of ruminal Firmicutes in vitro.
PG  - 1998-2010
LID - 10.1111/j.1365-2672.2010.04829.x [doi]
AB  - AIMS: To investigate the effect of the forage source and feed particle size (FPS) 
      in ruminant rations on the composition of the ruminal Firmicutes community in 
      vitro. METHODS AND RESULTS: Three diets, varying in maize silage to grass silage 
      ratio and FPS, were incubated in a rumen simulation system. Microbial samples 
      were taken from the liquid fermenter effluents. Microbial community analysis was 
      performed by 16S rRNA-based techniques. Clostridia-specific single-strand 
      conformation polymorphism profiles revealed changes of the community structure in 
      dependence on both factors tested. The coarse grass silage-containing diets 
      seemed to enhance the occurrence of different Roseburia species. As detected by 
      real-time quantitative PCR, Ruminococcus albus showed a higher abundance with 
      decreasing FPS. A slightly lower proportion of Bacilli was found with increasing 
      grass silage to maize silage ratio by fluorescence in situ hybridization (FISH). 
      In contrast, a slightly higher proportion of bacterial species belonging to the 
      Clostridium-clusters XIV a and b was detected by FISH with increasing grass 
      silage contents in the diet. CONCLUSIONS: The ruminal Firmicutes community is 
      affected by the choice of the forage source and FPS. SIGNIFICANCE AND IMPACT OF 
      THE STUDY: This study supplies fundamental knowledge about the response of 
      ruminal microbial communities to changing diets. Moreover, the data suggest a 
      standardization of grinding of feeds for in vitro studies to facilitate the 
      comparison of results of different laboratories.
CI  - (c) 2010 The Authors. Journal of Applied Microbiology (c) 2010 The Society for 
      Applied Microbiology.
FAU - Witzig, M
AU  - Witzig M
AD  - Universitat Hohenheim, Institut fur Tierernahrung, Stuttgart, Germany. 
      inst450@uni-hohenheim.de
FAU - Boguhn, J
AU  - Boguhn J
FAU - Kleinsteuber, S
AU  - Kleinsteuber S
FAU - Fetzer, I
AU  - Fetzer I
FAU - Rodehutscord, M
AU  - Rodehutscord M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - J Appl Microbiol
JT  - Journal of applied microbiology
JID - 9706280
RN  - 0 (DNA, Bacterial)
RN  - 0 (RNA, Ribosomal, 16S)
SB  - IM
MH  - Animals
MH  - Bacteria/genetics/*growth & development/isolation & purification
MH  - DNA, Bacterial/genetics
MH  - In Situ Hybridization, Fluorescence
MH  - Particle Size
MH  - Phylogeny
MH  - Poaceae
MH  - Polymorphism, Single-Stranded Conformational
MH  - RNA, Ribosomal, 16S/genetics
MH  - Rumen/*microbiology
MH  - Ruminants/*microbiology
MH  - Sequence Analysis, DNA
MH  - *Silage
MH  - Zea mays
EDAT- 2010/08/21 06:00
MHDA- 2011/03/22 06:00
CRDT- 2010/08/21 06:00
PHST- 2010/08/21 06:00 [entrez]
PHST- 2010/08/21 06:00 [pubmed]
PHST- 2011/03/22 06:00 [medline]
AID - JAM4829 [pii]
AID - 10.1111/j.1365-2672.2010.04829.x [doi]
PST - ppublish
SO  - J Appl Microbiol. 2010 Dec;109(6):1998-2010. doi: 
      10.1111/j.1365-2672.2010.04829.x.