PMID- 20723317
OWN - NLM
STAT- MEDLINE
DCOM- 20110930
LR  - 20161018
IS  - 1009-2137 (Print)
IS  - 1009-2137 (Linking)
VI  - 18
IP  - 4
DP  - 2010 Aug
TI  - [Establishment and evaluation of quantitative analysis method for donor chimerism 
      of mice].
PG  - 1002-6
AB  - This study was aimed to explore the effectiveness of sequential and quantitative 
      detection method for analysing donor chimerism (DC). In order to simulate a mouse 
      model of haploidentical stem cell transplantation, C57BL/6 male mice were used as 
      donors, while CB6F1 female mice were used as recipients and were divided into 2 
      groups. The two groups of recipients were irradiated with 2 Gy and 6 Gy from 
      ((6)(0)CO gamma-ray source respectively, and then were inoculated intravenously with 
      bone marrow cells (BMCs) and spleen mononuclear cells (SPMNCs). Quantitative 
      analyses of DC were performed with real-time PCR or flow cytometry (FCM) on 
      different days after transplantation. The results showed that real-time PCR and 
      FCM both have advantages and disadvantages in the detection of DC. When DC amount 
      in group of 6 Gy was > 90% with stable macrochimerism for more than 3 months, the 
      efficacy of detection by FCM was well; while the DC amount in group of 2 Gy was 
      below 10% and gradually transformed to different forms of microchimerism until 
      disappeared, in which condition the use of real time-PCR was more appropriate. It 
      is concluded that FCM can detect macrochimerism with high accuracy but would fail 
      when DC amount is less than 1% due to sensitivity limitation, while the real 
      time-PCR is more sensitive for detecting microchimerism but lack of accuracy for 
      detecting macrochimerism. Combination of the two methods can afford sensitive and 
      accurate tool for quantitative analysis of chimerism in mouse model of 
      transplantation and adoptive immunotherapy.
FAU - Zuo, Hong-Li
AU  - Zuo HL
AD  - Department of Hematology, 307th Hospital Affiliated to Academy of Military 
      Medical Sciences, Beijing 10007l, China.
FAU - Wang, Dan-Hong
AU  - Wang DH
FAU - Ai, Hui-Sheng
AU  - Ai HS
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Shi Yan Xue Ye Xue Za Zhi
JT  - Zhongguo shi yan xue ye xue za zhi
JID - 101084424
SB  - IM
MH  - Animals
MH  - *Bone Marrow Transplantation
MH  - Female
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Polymerase Chain Reaction/methods
MH  - *Transplantation Chimera
MH  - Transplantation, Homologous
EDAT- 2010/08/21 06:00
MHDA- 2011/10/01 06:00
CRDT- 2010/08/21 06:00
PHST- 2010/08/21 06:00 [entrez]
PHST- 2010/08/21 06:00 [pubmed]
PHST- 2011/10/01 06:00 [medline]
AID - 1009-2137(2010)04-1002-05 [pii]
PST - ppublish
SO  - Zhongguo Shi Yan Xue Ye Xue Za Zhi. 2010 Aug;18(4):1002-6.