PMID- 20723584
OWN - NLM
STAT- MEDLINE
DCOM- 20110329
LR  - 20101115
IS  - 1873-6971 (Electronic)
IS  - 0367-326X (Linking)
VI  - 81
IP  - 8
DP  - 2010 Dec
TI  - SCAR markers: a potential tool for authentication of herbal drugs.
PG  - 969-76
LID - 10.1016/j.fitote.2010.08.002 [doi]
AB  - Randomly Amplified Polymorphic DNA (RAPD) is easy to develop and simple molecular 
      marker, but lack of reproducibility makes it less reliable for authentication of 
      herbal drugs. Besides RAPD, other popular PCR and non-PCR based markers like 
      AFLP, ISSR, SSR and RFLP are also used for authentication. However, these also 
      have disadvantages like use of radioactive isotopes, high cost and absolute 
      requirement of sequence information. Therefore, it is a better option to improve 
      the reproducibility of RAPD by converting RAPD amplicons into Sequence 
      Characterized Amplified Region (SCAR) markers. SCAR markers are easy, reliable 
      and reproducible thus, have an advantage over RAPD markers for authentication of 
      medicinal herbs used in the preparation of traditional medicines. These markers 
      however, have been developed for only a few medicinal herbs. This review is an 
      attempt to evaluate critically the role of SCAR markers in authentication of 
      medicinal herbs used in traditional formulations.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Kiran, Usha
AU  - Kiran U
AD  - Department of Biotechnology, Faculty of Science, Centre for Transgenic Plant 
      Development, Jamia Hamdard, New Delhi 110062, India.
FAU - Khan, Salim
AU  - Khan S
FAU - Mirza, Khanda Jabeen
AU  - Mirza KJ
FAU - Ram, Mauji
AU  - Ram M
FAU - Abdin, M Z
AU  - Abdin MZ
LA  - eng
PT  - Journal Article
DEP - 20100817
PL  - Netherlands
TA  - Fitoterapia
JT  - Fitoterapia
JID - 16930290R
RN  - 0 (Genetic Markers)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - DNA/*analysis
MH  - *Genetic Markers
MH  - Plants, Medicinal/*genetics
MH  - Random Amplified Polymorphic DNA Technique
MH  - Species Specificity
EDAT- 2010/08/21 06:00
MHDA- 2011/03/30 06:00
CRDT- 2010/08/21 06:00
PHST- 2010/05/24 00:00 [received]
PHST- 2010/08/06 00:00 [revised]
PHST- 2010/08/06 00:00 [accepted]
PHST- 2010/08/21 06:00 [entrez]
PHST- 2010/08/21 06:00 [pubmed]
PHST- 2011/03/30 06:00 [medline]
AID - S0367-326X(10)00203-0 [pii]
AID - 10.1016/j.fitote.2010.08.002 [doi]
PST - ppublish
SO  - Fitoterapia. 2010 Dec;81(8):969-76. doi: 10.1016/j.fitote.2010.08.002. Epub 2010 
      Aug 17.