PMID- 20725669
OWN - NLM
STAT- MEDLINE
DCOM- 20110120
LR  - 20151119
IS  - 1364-5528 (Electronic)
IS  - 0003-2654 (Linking)
VI  - 135
IP  - 10
DP  - 2010 Oct
TI  - Simultaneous analysis of mercury and selenium species including chiral forms of 
      selenomethionine in human urine and serum by HPLC column-switching coupled to 
      ICP-MS.
PG  - 2700-5
LID - 10.1039/c0an00090f [doi]
AB  - The simultaneous speciation of elements is of great concern, especially in the 
      study of the interactions of species in living organisms. Here we report a method 
      based on the coupling of HPLC-ICP-MS that is capable of separating and analyzing 
      different selenium and mercury species (Se-methylselenocysteine, selenite, 
      selenate, L-selenomethionine, D-selenomethionine, methylmercury and inorganic 
      mercury). The proposed method uses two different mobile phases that are suitable 
      for selenium and mercury speciation and leads to a successful determination of 
      all the species in less than 27 min with good efficiency and resolution. The 
      method was efficiently applied for simultaneous speciation of mercury and 
      selenium in urine and in serum, the latter from umbilical cord samples. 
      Selenocystine has been successfully identified in the former sample. Detection 
      limits obtained were between 0.30 and 2.46 ng. Recovery studies of samples spiked 
      with all species were performed to check the reliability of the method, and 
      satisfactory recoveries (93-110%) were obtained in all cases. The relative 
      standard deviations (RSDs) for species with ten replicate determinations of 80 mug 
      L(-1) were between 4.5 and 9.2%. The proposed method offers a deeper insight into 
      selenium and mercury interactions in the human body.
FAU - Moreno, F
AU  - Moreno F
AD  - Departamento de Quimica y Ciencia de los Materiales, Facultad de Ciencias 
      Experimentales, Universidad de Huelva, Campus de El Carmen, 21007 Huelva, Spain.
FAU - Garcia-Barrera, T
AU  - Garcia-Barrera T
FAU - Gomez-Ariza, J L
AU  - Gomez-Ariza JL
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100820
PL  - England
TA  - Analyst
JT  - The Analyst
JID - 0372652
RN  - 0 (Methylmercury Compounds)
RN  - 0 (Organoselenium Compounds)
RN  - 0 (Selenium Compounds)
RN  - 0CH9049VIS (Selenocysteine)
RN  - 1464-43-3 (selenocystine)
RN  - 48TCX9A1VT (Cystine)
RN  - 964MRK2PEL (Selenomethionine)
RN  - FXS1BY2PGL (Mercury)
RN  - H6241UJ22B (Selenium)
RN  - HIW548RQ3W (Sodium Selenite)
RN  - HV0Y51NC4J (Selenic Acid)
RN  - K848JZ4886 (Cysteine)
RN  - TWK220499Z (selenomethylselenocysteine)
SB  - IM
MH  - Chromatography, High Pressure Liquid/*methods
MH  - Cysteine/analogs & derivatives/blood/urine
MH  - Cystine/analogs & derivatives/blood
MH  - Dietary Supplements
MH  - Humans
MH  - Mass Spectrometry/*methods
MH  - Mercury/*analysis/blood/urine
MH  - Methylmercury Compounds/blood/urine
MH  - Organoselenium Compounds/blood/urine
MH  - Selenic Acid
MH  - Selenium/*analysis/blood/urine
MH  - Selenium Compounds/blood/urine
MH  - Selenocysteine/analogs & derivatives
MH  - Selenomethionine/*analysis/blood/urine
MH  - Sodium Selenite/blood/urine
MH  - Stereoisomerism
EDAT- 2010/08/21 06:00
MHDA- 2011/01/21 06:00
CRDT- 2010/08/21 06:00
PHST- 2010/08/21 06:00 [entrez]
PHST- 2010/08/21 06:00 [pubmed]
PHST- 2011/01/21 06:00 [medline]
AID - 10.1039/c0an00090f [doi]
PST - ppublish
SO  - Analyst. 2010 Oct;135(10):2700-5. doi: 10.1039/c0an00090f. Epub 2010 Aug 20.