PMID- 20725954
OWN - NLM
STAT- MEDLINE
DCOM- 20110113
LR  - 20220408
IS  - 1552-4981 (Electronic)
IS  - 1552-4973 (Linking)
VI  - 95
IP  - 1
DP  - 2010 Oct
TI  - Biofilm formation and composition on different implant materials in vivo.
PG  - 101-9
LID - 10.1002/jbm.b.31688 [doi]
AB  - Biofilm formation was evaluated on the following titanium and zirconia implants 
      in vivo: machined titanium (Ti-m), modified titanium (TiUnite), modified zirconia 
      (ZiUnite), machined alumina-toughened zirconia (ATZ-m), sandblasted 
      alumina-toughened zirconia (ATZ-s), and machined zirconia (TZP-A-m). Bovine 
      enamel slabs were used as controls. Surface morphologies were examined by atomic 
      force (AFM) and scanning electron microscopy (SEM). The surface wettability was 
      also determined. Twelve healthy volunteers wore a splint system with the tested 
      materials. After 3 and 5 days the materials were examined by fluorescence in situ 
      hybridization (FISH) and confocal laser scanning microscopy (CLSM). The levels of 
      Streptococcus spp., Veillonella spp., Fusobacteriaum nucleatum, and Actinomyces 
      naeslundii were quantitatively determined. The biofilm thickness was found to be 
      between 19.78 and 36.73 mum after 3 days and between 26.11 and 32.43 mum after 5 
      days. With the exception of Ti-m the biofilm thickness after 3 days was 
      correlated with surface roughness. In addition to Streptococcus spp. as the main 
      component of the biofilm (11.23-25.30%), F. nucleatum, A. naeslundii, and 
      Veillonella spp. were also detected. No significant differences in biofilm 
      composition on the implant surfaces could be observed. In total, the influence of 
      roughness and material on biofilm formation was compensated by biofilm 
      maturation.
FAU - Al-Ahmad, A
AU  - Al-Ahmad A
AD  - Department of Operative Dentistry and Periodontology, Albert-Ludwigs-University, 
      79106 Freiburg, Germany.
FAU - Wiedmann-Al-Ahmad, M
AU  - Wiedmann-Al-Ahmad M
FAU - Faust, J
AU  - Faust J
FAU - Bachle, M
AU  - Bachle M
FAU - Follo, M
AU  - Follo M
FAU - Wolkewitz, M
AU  - Wolkewitz M
FAU - Hannig, C
AU  - Hannig C
FAU - Hellwig, E
AU  - Hellwig E
FAU - Carvalho, C
AU  - Carvalho C
FAU - Kohal, R
AU  - Kohal R
LA  - eng
PT  - Journal Article
PL  - United States
TA  - J Biomed Mater Res B Appl Biomater
JT  - Journal of biomedical materials research. Part B, Applied biomaterials
JID - 101234238
RN  - C6V6S92N3C (Zirconium)
RN  - D1JT611TNE (Titanium)
RN  - S38N85C5G0 (zirconium oxide)
SB  - IM
MH  - Animals
MH  - Bacteria/growth & development/*isolation & purification
MH  - Biofilms/*growth & development
MH  - Cattle
MH  - Humans
MH  - Materials Testing/methods
MH  - Prostheses and Implants/*microbiology
MH  - Surface Properties
MH  - Titanium
MH  - Young Adult
MH  - Zirconium
EDAT- 2010/08/21 06:00
MHDA- 2011/01/14 06:00
CRDT- 2010/08/21 06:00
PHST- 2010/08/21 06:00 [entrez]
PHST- 2010/08/21 06:00 [pubmed]
PHST- 2011/01/14 06:00 [medline]
AID - 10.1002/jbm.b.31688 [doi]
PST - ppublish
SO  - J Biomed Mater Res B Appl Biomater. 2010 Oct;95(1):101-9. doi: 
      10.1002/jbm.b.31688.