PMID- 20726505
OWN - NLM
STAT- MEDLINE
DCOM- 20110104
LR  - 20131121
IS  - 1520-6882 (Electronic)
IS  - 0003-2700 (Linking)
VI  - 82
IP  - 18
DP  - 2010 Sep 15
TI  - Utilizing three monoclonal antibodies in the development of an 
      immunochromatographic assay for simultaneous detection of sulfamethazine, 
      sulfadiazine, and sulfaquinoxaline residues in egg and chicken muscle.
PG  - 7550-5
LID - 10.1021/ac101020y [doi]
AB  - A rapid and sensitive immunochromatographic assay (ICA) based on competitive 
      format was developed and validated for simultaneous detection of sulfamethazine 
      (SM(2)), sulfadiazine (SDZ), and sulfaquinoxaline (SQX) in chicken breast muscle 
      and egg samples. For this purpose, three monoclonal antibodies raised against 
      those three sulfonamides were conjugated to colloidal gold particles and applied 
      to the conjugate pads of the test strip. The competitors of the sulfonamides 
      (SM(2)/SDZ/SQX-bovine serum albumin conjugates) were immobilized onto a 
      nitrocellulose membrane at three detection zones to form T(1), T(2), and T(3), 
      respectively. With this method, the cutoff values for the three test lines were 
      achieved at 80 mug/kg, which is lower than the maximum residue levels (MRLs) 
      established for sulfonamides. The recoveries in negative samples spiked at 
      concentrations of 10, 50, and 100 mug/kg ranged from 75% to 82% for egg samples 
      and from 78% to 81% for chicken samples. The method was compared with the HPLC 
      method by testing 180 eggs and chicken breast samples from local markets, and an 
      agreement rate of 99.7% was obtained between the two methods.
FAU - Guo, Yancheng
AU  - Guo Y
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Wuhan 430070, P.R. China.
FAU - Ngom, Babacar
AU  - Ngom B
FAU - Le, Tao
AU  - Le T
FAU - Jin, Xiue
AU  - Jin X
FAU - Wang, Liping
AU  - Wang L
FAU - Shi, Deshi
AU  - Shi D
FAU - Wang, Xiliang
AU  - Wang X
FAU - Bi, Dingren
AU  - Bi D
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Anal Chem
JT  - Analytical chemistry
JID - 0370536
RN  - 0 (Antibodies, Monoclonal)
RN  - 0 (Gold Colloid)
RN  - 0N7609K889 (Sulfadiazine)
RN  - 48U51W007F (Sulfamethazine)
RN  - WNW8115TM9 (Sulfaquinoxaline)
SB  - IM
MH  - Analytic Sample Preparation Methods
MH  - Animals
MH  - Antibodies, Monoclonal/*immunology
MH  - Cattle
MH  - Chickens
MH  - Chromatography/*methods
MH  - Chromatography, High Pressure Liquid
MH  - Drug Residues/*analysis
MH  - Food Contamination/*analysis
MH  - Gold Colloid/chemistry
MH  - Immunoassay/*methods
MH  - Meat/analysis
MH  - Muscles/*chemistry
MH  - Ovum/*chemistry
MH  - Sulfadiazine/analysis/immunology
MH  - Sulfamethazine/analysis/immunology
MH  - Sulfaquinoxaline/analysis/immunology
MH  - Time Factors
EDAT- 2010/08/24 06:00
MHDA- 2011/01/05 06:00
CRDT- 2010/08/24 06:00
PHST- 2010/08/24 06:00 [entrez]
PHST- 2010/08/24 06:00 [pubmed]
PHST- 2011/01/05 06:00 [medline]
AID - 10.1021/ac101020y [doi]
PST - ppublish
SO  - Anal Chem. 2010 Sep 15;82(18):7550-5. doi: 10.1021/ac101020y.