PMID- 20726834
OWN - NLM
STAT- MEDLINE
DCOM- 20101001
LR  - 20100910
IS  - 1532-4303 (Electronic)
IS  - 0277-0903 (Linking)
VI  - 47
IP  - 7
DP  - 2010 Sep
TI  - Component-resolved diagnosis for phleum allergy: the role of recombinants.
PG  - 750-3
LID - 10.3109/02770903.2010.489247 [doi]
AB  - BACKGROUND: Conventional diagnostic tests (such as radioallergosorbent test 
      [RAST] and skin prick test [SPT]) use native raw pollen allergen extracts to 
      establish allergy. However, recombinant allergens may offer important advantages 
      compared with their natural counterparts. OBJECTIVE: This study evaluated serum 
      immunoglobulin E (IgE) in patients with grass-induced allergic rhinitis (AR) or 
      AR with asthma (ARA), comparing assays with natural or recombinant grass 
      allergens. METHODS: Sixty patients (33 AR, 27 ARA) positive with SPT and serum 
      IgE for Phleum pratense were enrolled in the study. Serum IgE specific for 
      conventional and recombinant Phleum pratense: rPhl p 1, rPhl p 2, nPhl p 4, rPhl 
      5b, rPhl p 6, rPhl p 7, rPhl p 11, rPhl p 12, were measured by the IFMA procedure 
      (ImmunoCAP, Phadia, Uppsala, Sweden). Data were expressed as the median (md) and 
      percentiles. Recombinant allergen results were expressed also as the percentage 
      of positive concentrations. The Wilcoxon test was used to compare samples. 
      Because diagnosis is a binary variable (AR/ARA), logistic regression analysis was 
      performed to identify possible correlates. RESULTS: IgE concentrations assessed 
      with recombinant allergens were significantly higher in ARA patients (p = .05) 
      than in AR patients. A value >5.8 kU/L is the optimal cut-off to discriminate AR 
      and ARA patients. Model specificity was 76%, sensitivity 78%, and efficiency 77%. 
      CONCLUSION: This study shows that IgEs for natural and recombinant grass pollen 
      allergens are significantly higher in patients with AR and asthma. Moreover, 
      using recombinant allergens it is possible to define a prediction model for 
      diagnosis with 77% efficiency. Therefore, this study may suggest that there are 
      advantages of using recombinant or purified, native allergens over crude 
      extracts.
FAU - De Amici, Mara
AU  - De Amici M
AD  - Department of Pediatric Science, Pediatric Clinic, Foundation IRCCS San Matteo, 
      Pavia, Italy.
FAU - Alesina, Roberta
AU  - Alesina R
FAU - Moratti, Remigio
AU  - Moratti R
FAU - Ciprandi, Giorgio
AU  - Ciprandi G
LA  - eng
PT  - Journal Article
PL  - England
TA  - J Asthma
JT  - The Journal of asthma : official journal of the Association for the Care of 
      Asthma
JID - 8106454
RN  - 0 (Allergens)
RN  - 0 (Recombinant Proteins)
RN  - 37341-29-0 (Immunoglobulin E)
SB  - IM
MH  - Adult
MH  - Allergens/*immunology
MH  - Asthma/*diagnosis
MH  - Female
MH  - Humans
MH  - Immunoglobulin E/blood
MH  - Male
MH  - Multivariate Analysis
MH  - Phleum/*immunology
MH  - ROC Curve
MH  - Recombinant Proteins/immunology
MH  - Rhinitis, Allergic, Seasonal/*diagnosis
EDAT- 2010/08/24 06:00
MHDA- 2010/10/05 06:00
CRDT- 2010/08/24 06:00
PHST- 2010/08/24 06:00 [entrez]
PHST- 2010/08/24 06:00 [pubmed]
PHST- 2010/10/05 06:00 [medline]
AID - 10.3109/02770903.2010.489247 [doi]
PST - ppublish
SO  - J Asthma. 2010 Sep;47(7):750-3. doi: 10.3109/02770903.2010.489247.