PMID- 20727526
OWN - NLM
STAT- MEDLINE
DCOM- 20101202
LR  - 20131121
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1217
IP  - 39
DP  - 2010 Sep 24
TI  - Development and validation of a liquid chromatography tandem mass spectrometry 
      method for the analysis of beta-agonists in animal feed and drinking water.
PG  - 6061-8
LID - 10.1016/j.chroma.2010.07.034 [doi]
AB  - A reproducible, sensitive and selective multiresidue analytical method for seven 
      beta-agonists: clenbuterol (CBT), clenpenterol (CPT), ractopamine (RTP), 
      brombuterol (BBT), mabuterol (MBT), mapenterol (MPT), and 
      hydroxymethylclenbuterol (HMCBT) was developed and validated by using liquid 
      chromatography tandem mass spectrometry (LC-MS/MS) in feed and drinking water 
      samples. The validation was achieved according to the criteria laid down in the 
      Commission Decision 2002/657/EC, however it was necessary to use minimum required 
      performance limits (MRPLs) proposed by the Community Reference Laboratories 
      (CRLs) due to the lack of maximum residue limits (MRLs) for beta-agonists. By 
      setting up these MRPLs, allows controlling their use in safe mode, since 
      beta-agonists are commonly used in veterinary medicine sometime in a fraudulent 
      manner, for increasing the weigh of animals. Values set for both matrices studied 
      are 50 microg/kg for animal feed, and a range from 0.2 to 10 microg/L for 
      drinking water. CCalpha values calculated were under the MRPLs suggested; for 
      drinking water the lowest value obtained was 0.12 microg/L, and for animal feed 
      0.87 microg/kg. Values for CCbeta were ranged from 0.08 to 0.13 microg/L in 
      drinking water and from 0.5 to 0.92 microg/kg in animal feed samples. The 
      excellence values obtained, allowed us to conclude that the proposed analytical 
      method is capable to control the beta-agonists studied in both matrices and that 
      it can be successfully applied and used as a routine method in laboratories of 
      residue analysis of veterinary food control.
CI  - Copyright 2010 Elsevier B.V. All rights reserved.
FAU - Juan, C
AU  - Juan C
AD  - Centro Superior de Investigacion en Salud Publica, Unidad de Residuos de 
      Medicamentos Veterinarios, Laboratorio de Salud Publica de Valencia, Generalitat 
      Valenciana, Av. de Cataluna n degrees 21, 46020 Valencia, Spain. juan_cri@gva.es
FAU - Igualada, C
AU  - Igualada C
FAU - Moragues, F
AU  - Moragues F
FAU - Leon, N
AU  - Leon N
FAU - Manes, J
AU  - Manes J
LA  - eng
PT  - Journal Article
DEP - 20100805
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Adrenergic beta-Agonists)
RN  - 0 (Ethanolamines)
RN  - 0 (Phenethylamines)
RN  - 059QF0KO0R (Water)
RN  - 57370OZ3P1 (ractopamine)
SB  - IM
MH  - Adrenergic beta-Agonists/*analysis
MH  - Animal Feed/*analysis
MH  - Chromatography, Liquid/*methods
MH  - Ethanolamines/analysis
MH  - Linear Models
MH  - Phenethylamines/analysis
MH  - Reproducibility of Results
MH  - Sensitivity and Specificity
MH  - Tandem Mass Spectrometry/*methods
MH  - Water/chemistry
MH  - Water Supply/*analysis
EDAT- 2010/08/24 06:00
MHDA- 2010/12/14 06:00
CRDT- 2010/08/24 06:00
PHST- 2010/04/08 00:00 [received]
PHST- 2010/06/18 00:00 [revised]
PHST- 2010/07/13 00:00 [accepted]
PHST- 2010/08/24 06:00 [entrez]
PHST- 2010/08/24 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
AID - S0021-9673(10)00954-4 [pii]
AID - 10.1016/j.chroma.2010.07.034 [doi]
PST - ppublish
SO  - J Chromatogr A. 2010 Sep 24;1217(39):6061-8. doi: 10.1016/j.chroma.2010.07.034. 
      Epub 2010 Aug 5.