PMID- 20728584
OWN - NLM
STAT- MEDLINE
DCOM- 20110214
LR  - 20161125
IS  - 1878-7568 (Electronic)
IS  - 1742-7061 (Linking)
VI  - 7
IP  - 1
DP  - 2011 Jan
TI  - Surface modification of electrospun PLLA nanofibers by plasma treatment and 
      cationized gelatin immobilization for cartilage tissue engineering.
PG  - 234-43
LID - 10.1016/j.actbio.2010.08.015 [doi]
AB  - Electrospun poly(lactic acid) (PLLA) nanofibers (NF) were modified with 
      cationized gelatin (CG) to improve their compatibility with chondrocytes and to 
      show in vitro and in vivo the potential applications of CG-grafted PLLA 
      nanofibrous membranes (CG-PLLA NFM) as a cartilage tissue engineering scaffold. 
      PLLA NF were first treated with oxygen plasma to introduce -COOH groups on the 
      surface, followed by covalent grafting of CG molecules onto the fiber surface, 
      using water-soluble carbodiimide as the coupling agent. The effects of CG 
      grafting and properties of NFM were characterized by scanning electron microscopy 
      (SEM), transmission electron microscopy, thermogravimetric analysis, atomic force 
      microscope, X-ray photoelectron spectra and Fourier transform infrared 
      spectroscopy. In vitro studies indicated that CG-PLLA NFM could enhance 
      viability, proliferation and differentiation of rabbit articular chondrocytes 
      compared with pristine PLLA NFM. SEM observations of the cell-scaffold construct 
      confirmed the tight attachment of chondrocytes to CG-PLLA NF and in-growth of 
      cells into the interior of the membrane with proper maintenance of cell 
      morphology. Improved cell differentiation in CG-PLLA NFM was confirmed by 
      enhanced glycoaminoglycan and collagen secretion, histological analysis and 
      reverse transcription-polymerase chain reaction studies, which showed that the 
      cells were able to maintain the expression of characteristic markers (collagen 
      II, aggregan and SOX 9) of chondrocytes. Subcutaneous implantation of the 
      cell-scaffold constructs with autologous chondrocytes also confirmed the 
      formation of ectopic cartilage tissues after 28 days by histological examination 
      and immunostaining.
CI  - Copyright (c) 2010 Acta Materialia Inc. Published by Elsevier Ltd. All rights 
      reserved.
FAU - Chen, Jyh-Ping
AU  - Chen JP
AD  - Department of Chemical and Materials Engineering, Chang Gung University, 
      Kwei-San, Taoyuan, Taiwan, ROC. jpchen@mail.cgu.edu.tw
FAU - Su, Chien-Hao
AU  - Su CH
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100820
PL  - England
TA  - Acta Biomater
JT  - Acta biomaterialia
JID - 101233144
RN  - 0 (Cations)
RN  - 0 (Immobilized Proteins)
RN  - 0 (Membranes, Artificial)
RN  - 0 (Plasma Gases)
RN  - 0 (Polyesters)
RN  - 0 (Polymers)
RN  - 33X04XA5AT (Lactic Acid)
RN  - 459TN2L5F5 (poly(lactide))
RN  - 9000-70-8 (Gelatin)
SB  - IM
MH  - Animals
MH  - Cartilage/cytology/drug effects/*physiology
MH  - Cations
MH  - Cell Proliferation/drug effects
MH  - Cell Shape/drug effects
MH  - Cell Survival/drug effects
MH  - Chondrocytes/cytology/drug effects/metabolism/ultrastructure
MH  - Extracellular Matrix/drug effects/genetics/metabolism
MH  - Gelatin/*pharmacology
MH  - Gene Expression Regulation/drug effects
MH  - Immobilized Proteins/*pharmacology
MH  - Immunohistochemistry
MH  - Lactic Acid/*pharmacology
MH  - Membranes, Artificial
MH  - Nanofibers/*chemistry/ultrastructure
MH  - Photoelectron Spectroscopy
MH  - Plasma Gases/*chemistry
MH  - Polyesters
MH  - Polymers/*pharmacology
MH  - Rabbits
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Surface Properties/drug effects
MH  - Sus scrofa
MH  - Thermogravimetry
MH  - Tissue Engineering/*methods
EDAT- 2010/08/24 06:00
MHDA- 2011/02/15 06:00
CRDT- 2010/08/24 06:00
PHST- 2010/05/15 00:00 [received]
PHST- 2010/08/11 00:00 [revised]
PHST- 2010/08/16 00:00 [accepted]
PHST- 2010/08/24 06:00 [entrez]
PHST- 2010/08/24 06:00 [pubmed]
PHST- 2011/02/15 06:00 [medline]
AID - S1742-7061(10)00385-5 [pii]
AID - 10.1016/j.actbio.2010.08.015 [doi]
PST - ppublish
SO  - Acta Biomater. 2011 Jan;7(1):234-43. doi: 10.1016/j.actbio.2010.08.015. Epub 2010 
      Aug 20.