PMID- 20729070
OWN - NLM
STAT- MEDLINE
DCOM- 20110207
LR  - 20100927
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 26
IP  - 2
DP  - 2010 Oct 15
TI  - Direct fluorescence in situ hybridization (FISH) in Escherichia coli with a 
      target-specific quantum dot-based molecular beacon.
PG  - 491-6
LID - 10.1016/j.bios.2010.07.067 [doi]
AB  - Quantum dots (QDs) are inorganic fluorescent nanocrystals with excellent 
      properties such as tunable emission spectra and photo-bleaching resistance 
      compared with organic dyes, which make them appropriate for applications in 
      molecular beacons. In this work, quantum dot-based molecular beacons (QD-based 
      MBs) were fabricated to specifically detect beta-lactamase genes located in pUC18 
      which were responsible for antibiotic resistance in bacteria Escherichia coli (E. 
      coli) DH5alpha. QD-based MBs were constructed by conjugating mercaptoacetic 
      acid-quantum dots (MAA-QDs) with black hole quencher 2 (BHQ2) labeled thiol DNA 
      vial metal-thiol bonds. Two types of molecular beacons, double-strands beacons 
      and hairpin beacons, were observed in product characterization by gel 
      electrophoresis. Using QD-based MBs, one-step FISH in tiny bacteria DH5alpha was 
      realized for the first time. QD-based MBs retained their bioactivity when 
      hybridizing with complementary target DNA, which showed excellent advantages of 
      eliminating background noise caused by adsorption of non-specific bioprobes and 
      achieving clearer focus of genes in plasmids pUC18, and capability of bacterial 
      cell penetration and signal specificity in one-step in situ hybridization.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Wu, Sheng-Mei
AU  - Wu SM
AD  - Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of 
      Education), College of Chemistry and Molecular Sciences, State Key Laboratory of 
      Virology, Wuhan University, Wuhan 430072, PR China.
FAU - Tian, Zhi-Quan
AU  - Tian ZQ
FAU - Zhang, Zhi-Ling
AU  - Zhang ZL
FAU - Huang, Bi-Hai
AU  - Huang BH
FAU - Jiang, Peng
AU  - Jiang P
FAU - Xie, Zhi-Xiong
AU  - Xie ZX
FAU - Pang, Dai-Wen
AU  - Pang DW
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100724
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 0 (DNA Probes)
RN  - EC 3.5.2.6 (beta-Lactamases)
SB  - IM
MH  - DNA Probes/*genetics
MH  - Escherichia coli/*genetics
MH  - Gene Targeting/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - *Molecular Probe Techniques
MH  - *Quantum Dots
MH  - beta-Lactamases/*genetics
EDAT- 2010/08/24 06:00
MHDA- 2011/02/08 06:00
CRDT- 2010/08/24 06:00
PHST- 2010/05/05 00:00 [received]
PHST- 2010/07/16 00:00 [revised]
PHST- 2010/07/16 00:00 [accepted]
PHST- 2010/08/24 06:00 [entrez]
PHST- 2010/08/24 06:00 [pubmed]
PHST- 2011/02/08 06:00 [medline]
AID - S0956-5663(10)00442-2 [pii]
AID - 10.1016/j.bios.2010.07.067 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2010 Oct 15;26(2):491-6. doi: 10.1016/j.bios.2010.07.067. 
      Epub 2010 Jul 24.