PMID- 20729266
OWN - NLM
STAT- MEDLINE
DCOM- 20110908
LR  - 20220318
IS  - 1460-2385 (Electronic)
IS  - 0931-0509 (Linking)
VI  - 26
IP  - 3
DP  - 2011 Mar
TI  - Toll-like receptor expression in monocytes in patients with chronic kidney 
      disease and haemodialysis: relation with inflammation.
PG  - 955-63
LID - 10.1093/ndt/gfq500 [doi]
AB  - BACKGROUND: Inflammation is one of the main contributors to atherosclerosis in 
      haemodialysis (HD) patients. Activation of Toll-like receptors (TLRs) leads to 
      inflammatory response. In this study, we aimed to evaluate the expression of TLRs 
      on monocytes and relate their expression with inflammation in chronic kidney 
      disease (CKD) and HD patients. METHODS: Thirty-four age- and gender-matched 
      controls and stage 3-4 CKD patients and thirty-two HD patients were included in 
      each study group. The effect of HD on the expression of Toll-like receptor-2 
      (TLR-2) and Toll-like receptor-4 (TLR-4) on CD14( +) monocytes was determined at 
      the beginning (baseline), during (120 min) and following (300 min and 24 h) HD 
      and compared with control and stage 3-4 CKD groups. The HD procedure was 
      performed by using low-flux polysulphone dialysers. In addition, serum IL-6 
      levels were evaluated in both groups at baseline and after a HD session. RESULTS: 
      The percentage of CD14( +) monocytes expressing TLR-2 were similar in all of the 
      study groups, whereas the percentage of CD14( +) monocytes expressing TLR-4 were 
      significantly lower in both stage 3-4 CKD and HD patients at baseline than in 
      controls. The mean fluorescence intensities (MFI) of TLR-2 were significantly 
      lower in controls than in stage 3-4 CKD and HD patients at baseline. The MFI of 
      TLR-4 was similar in all of the groups. The percentage of CD14( +) monocytes 
      expressing TLR-2 did not change during and after HD. The MFI of TLR-2 decreased 
      at 120 min of HD compared with baseline (1837 +/- 672 vs 1650 +/- 578, P < 0.05), and 
      recovered back to baseline values at 300 min and at 24 h post-HD. MFI of TLR-4 
      increased at 24 h compared with baseline (941 +/- 294 vs 1087 +/- 441, P < 0.05). 
      Serum IL-6 levels correlated with MFI of TLR-2 and TLR-4 in stage 3-4 CKD 
      patients and in HD patients at baseline and after HD in univariate analysis. 
      Stepwise multiple regression analysis revealed that MFI of TLR-2 was an 
      independent determinant of serum IL-6 concentrations in stage 3-4 CKD and in HD 
      patients at baseline, at 300 min and at 24 h post-HD. Conclusions. Our study 
      demonstrates that TLR-2 is associated with the inflammatory response of 
      non-dialysed and dialysed CKD patients.
FAU - Koc, Mehmet
AU  - Koc M
AD  - Department of Internal Medicine, Division of Nephrology Marmara University School 
      of Medicine, Istanbul, Turkey. drmkoc@yahoo.com
FAU - Toprak, Ahmet
AU  - Toprak A
FAU - Arikan, Hakki
AU  - Arikan H
FAU - Odabasi, Zekaver
AU  - Odabasi Z
FAU - Elbir, Yesim
AU  - Elbir Y
FAU - Tulunay, Aysin
AU  - Tulunay A
FAU - Asicioglu, Ebru
AU  - Asicioglu E
FAU - Eksioglu-Demiralp, Emel
AU  - Eksioglu-Demiralp E
FAU - Glorieux, Griet
AU  - Glorieux G
FAU - Vanholder, Raymond
AU  - Vanholder R
FAU - Akoglu, Emel
AU  - Akoglu E
LA  - eng
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100820
PL  - England
TA  - Nephrol Dial Transplant
JT  - Nephrology, dialysis, transplantation : official publication of the European 
      Dialysis and Transplant Association - European Renal Association
JID - 8706402
RN  - 0 (Interleukin-6)
RN  - 0 (TLR2 protein, human)
RN  - 0 (TLR4 protein, human)
RN  - 0 (Toll-Like Receptor 2)
RN  - 0 (Toll-Like Receptor 4)
SB  - IM
MH  - Case-Control Studies
MH  - Female
MH  - Flow Cytometry
MH  - Glomerular Filtration Rate
MH  - Humans
MH  - Inflammation/*etiology/metabolism
MH  - Interleukin-6/blood
MH  - Kidney Failure, Chronic/*metabolism
MH  - Kidney Function Tests
MH  - Male
MH  - Middle Aged
MH  - Monocytes/*metabolism
MH  - Prognosis
MH  - *Renal Dialysis
MH  - Risk Factors
MH  - Toll-Like Receptor 2/*metabolism
MH  - Toll-Like Receptor 4/*metabolism
EDAT- 2010/08/24 06:00
MHDA- 2011/09/09 06:00
CRDT- 2010/08/24 06:00
PHST- 2010/08/24 06:00 [entrez]
PHST- 2010/08/24 06:00 [pubmed]
PHST- 2011/09/09 06:00 [medline]
AID - gfq500 [pii]
AID - 10.1093/ndt/gfq500 [doi]
PST - ppublish
SO  - Nephrol Dial Transplant. 2011 Mar;26(3):955-63. doi: 10.1093/ndt/gfq500. Epub 
      2010 Aug 20.