PMID- 20729332
OWN - NLM
STAT- MEDLINE
DCOM- 20101123
LR  - 20230425
IS  - 1550-6606 (Electronic)
IS  - 0022-1767 (Print)
IS  - 0022-1767 (Linking)
VI  - 185
IP  - 6
DP  - 2010 Sep 15
TI  - Route of antigen uptake differentially impacts presentation by dendritic cells 
      and activated monocytes.
PG  - 3426-35
LID - 10.4049/jimmunol.1001205 [doi]
AB  - Dendritic cells (DCs), which maintain tolerance and orchestrate T cell immune 
      responses, comprise a heterogeneous group of cells. For example, in the steady 
      state, murine spleen contains pre-DC-derived CD8(+) and CD8(-) conventional DCs. 
      During inflammation, monocytes become activated and acquire some DC-like 
      features, such as expression of CD11c and MHC class II. Although each of these 
      cell types can present Ag, the relative efficiency of processing and presentation 
      after Ag capture by different routes has not yet been systematically compared. To 
      this end, we administered OVA to various conventional DCs and activated monocytes 
      by receptor-mediated endocytosis, pinocytosis, or phagocytosis and measured 
      internalization and presentation to MHC class I- and MHC class II-restricted T 
      cells. We find that CD8(-) DCs are more efficient than any other type of APC 
      tested in terms of presenting Ag to MHC class II-restricted T cells, irrespective 
      of the route of Ag capture. In contrast, both subsets of splenic DCs are highly 
      effective in cross-presenting Ags to CD8(+) T cells. DCs and activated monocytes 
      cross-presented Ags delivered by DEC205-mediated endocytosis and pinocytosis. 
      However, DCs differ from activated monocytes in that the latter are several 
      orders of magnitude less efficient in presenting Ags captured by phagocytosis to 
      CD8(+) or CD4(+) T cells. We conclude that DCs derived from pre-DCs differ from 
      monocyte-derived cells in that DCs process and present Ags efficiently 
      irrespective of the route of Ag capture. Our observations have significant 
      implications for understanding initiation of immune responses and vaccination 
      strategies targeting DCs and activated monocytes.
FAU - Kamphorst, Alice O
AU  - Kamphorst AO
AD  - Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 
      10065, USA.
FAU - Guermonprez, Pierre
AU  - Guermonprez P
FAU - Dudziak, Diana
AU  - Dudziak D
FAU - Nussenzweig, Michel C
AU  - Nussenzweig MC
LA  - eng
GR  - HHMI/Howard Hughes Medical Institute/United States
GR  - P01 AI051573/AI/NIAID NIH HHS/United States
GR  - AI051573/AI/NIAID NIH HHS/United States
PT  - Comparative Study
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100820
PL  - United States
TA  - J Immunol
JT  - Journal of immunology (Baltimore, Md. : 1950)
JID - 2985117R
RN  - 0 (Antigens, CD)
RN  - 0 (CD8 Antigens)
RN  - 0 (DEC-205 receptor)
RN  - 0 (Histocompatibility Antigens Class I)
RN  - 0 (Histocompatibility Antigens Class II)
RN  - 0 (Lectins, C-Type)
RN  - 0 (Minor Histocompatibility Antigens)
RN  - 0 (Receptors, Cell Surface)
SB  - IM
MH  - Animals
MH  - Antigens, CD/physiology
MH  - CD8 Antigens/metabolism/*physiology
MH  - CD8-Positive T-Lymphocytes/immunology/metabolism
MH  - Cells, Cultured
MH  - Cross-Priming/*immunology
MH  - Dendritic Cells/cytology/*immunology/metabolism
MH  - Endocytosis/immunology
MH  - Histocompatibility Antigens Class I/immunology/metabolism
MH  - Histocompatibility Antigens Class II/immunology/metabolism
MH  - Lectins, C-Type/physiology
MH  - Macrophage Activation/*immunology
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Mice, Knockout
MH  - Mice, Transgenic
MH  - Minor Histocompatibility Antigens
MH  - Monocytes/*immunology/metabolism
MH  - Receptors, Cell Surface/physiology
MH  - Resting Phase, Cell Cycle/immunology
PMC - PMC3013633
MID - NIHMS256220
OID - NLM: HHMIMS256220
EDAT- 2010/08/24 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/09/15
CRDT- 2010/08/24 06:00
PHST- 2010/08/24 06:00 [entrez]
PHST- 2010/08/24 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/09/15 00:00 [pmc-release]
AID - jimmunol.1001205 [pii]
AID - 10.4049/jimmunol.1001205 [doi]
PST - ppublish
SO  - J Immunol. 2010 Sep 15;185(6):3426-35. doi: 10.4049/jimmunol.1001205. Epub 2010 
      Aug 20.