PMID- 20729600 OWN - NLM STAT- MEDLINE DCOM- 20101116 LR - 20121115 IS - 0273-1223 (Print) IS - 0273-1223 (Linking) VI - 62 IP - 4 DP - 2010 TI - Biodegradation of 2,4-dichlorophenol in a fluidized bed reactor with immobilized Phanerochaete chrysosporium. PG - 947-55 LID - 10.2166/wst.2010.320 [doi] AB - The performance of a fluidized bed reactor using immobilized Phanerochaete chrysosporium to remove 2,4-dichlorophenol (2,4-DCP) from aqueous solution was investigated. The contribution of lignin peroxidase (LiP) and manganese peroxidase (MnP) secreted by Phanerochaete chrysosporium to the 2,4-DCP degradation was examined. Results showed that Lip and Mnp were not essential to 2,4-DCP degradation while their presence enhanced the degradation process and reaction rate. In sequential batch experiment, the bioactivity of immobilized cells was recovered and improved during the culture and the maximum degradation rate constant of 13.95 mg (Ld)(-)(1) could be reached. In continuous bioreactor test, the kinetic behavior of the Phanerochaete chrysosporium immobilized on loofa sponge was found to follow the Monod equation. The maximum reaction rate was 7.002 mg (Lh)(-)(1), and the saturation constant was 26.045 mg L(-)(1). FAU - Li, Xiao-ming AU - Li XM AD - Key Laboratory of Environmental Biology and Pollution Control (Hunan University), Ministry of Education, Changsha 410082, P.R. China. FAU - Yang, Qi AU - Yang Q FAU - Zhang, Ying AU - Zhang Y FAU - Zheng, Wei AU - Zheng W FAU - Yue, Xiu AU - Yue X FAU - Wang, Dong-bo AU - Wang DB FAU - Zeng, Guang-ming AU - Zeng GM LA - eng PT - Journal Article PT - Research Support, Non-U.S. Gov't PL - England TA - Water Sci Technol JT - Water science and technology : a journal of the International Association on Water Pollution Research JID - 9879497 RN - 0 (Chlorophenols) RN - 0 (Culture Media) RN - 0 (Fungal Proteins) RN - EC 1.11.1.- (Peroxidases) RN - EC 1.11.1.- (lignin peroxidase) RN - EC 1.11.1.13 (manganese peroxidase) RN - R669TG1950 (2,4-dichlorophenol) SB - IM MH - *Bioreactors MH - Biotransformation MH - Cells, Immobilized/*metabolism MH - Chlorophenols/isolation & purification/*metabolism/pharmacology MH - Culture Media MH - Fungal Proteins/metabolism MH - Peroxidases/metabolism MH - Phanerochaete/drug effects/*enzymology/growth & development EDAT- 2010/08/24 06:00 MHDA- 2010/11/17 06:00 CRDT- 2010/08/24 06:00 PHST- 2010/08/24 06:00 [entrez] PHST- 2010/08/24 06:00 [pubmed] PHST- 2010/11/17 06:00 [medline] AID - 10.2166/wst.2010.320 [doi] PST - ppublish SO - Water Sci Technol. 2010;62(4):947-55. doi: 10.2166/wst.2010.320.