PMID- 20730643
OWN - NLM
STAT- MEDLINE
DCOM- 20110127
LR  - 20191210
IS  - 1944-0057 (Electronic)
IS  - 1944-0057 (Linking)
VI  - 27
IP  - 11
DP  - 2010 Nov
TI  - UNE-EN ISO/IEC 17025:2005-accredited method for the determination of pesticide 
      residues in fruit and vegetable samples by LC-MS/MS.
PG  - 1532-44
LID - 10.1080/19440049.2010.506602 [doi]
AB  - A rapid, simple and sensitive multi-residue method was developed and validated 
      for the simultaneous quantification and confirmation of 69 pesticides in fruit 
      and vegetables using liquid chromatography-tandem mass spectrometry (LC-MS/MS). 
      The samples were extracted following the quick, easy, cheap, effective, rugged 
      and safe method known as QuEChERS. Mass spectrometric conditions were 
      individually optimised for each analyte in order to achieve maximum sensitivity 
      in multiple reaction monitoring (MRM) mode. Using the developed chromatographic 
      conditions, 69 pesticides can be separated in less than 17 min. Two selected 
      reaction monitoring (SRM) assays were used for each pesticide to obtain 
      simultaneous quantification and identification in one run. With this method in 
      SRM mode, more than 150 pesticides can be analysed and quantified, but their 
      confirmation is not possible in all cases according to the European regulations 
      on pesticide residues. Nine common representative matrices (zucchini, melon, 
      cucumber, watermelon, tomato, garlic, eggplant, lettuce and pepper) were selected 
      to investigate the effect of different matrices on recovery and precision. Mean 
      recoveries ranged from 70% to 120%, with relative standard deviations (RSDs) 
      lower than 20% for all the pesticides. The proposed method was applied to the 
      analysis of more than 2000 vegetable samples from the extensive greenhouse 
      cultivation in the province of Almeria, Spain, during one year. The methodology 
      combines the advantages of both QuEChERS and LC-MS/MS producing a very rapid, 
      sensitive, accurate and reliable procedure that can be applied in routine 
      analytical laboratories. The method was validated and accredited according to 
      UNE-EN-ISO/IEC 17025:2005 international standard (accreditation number 
      278/LE1027).
FAU - Camino-Sanchez, F J
AU  - Camino-Sanchez FJ
AD  - Laboratorios Cavendish S.L. Research Department, E-18220 Granada, Spain.
FAU - Zafra-Gomez, A
AU  - Zafra-Gomez A
FAU - Oliver-Rodriguez, B
AU  - Oliver-Rodriguez B
FAU - Ballesteros, O
AU  - Ballesteros O
FAU - Navalon, A
AU  - Navalon A
FAU - Crovetto, G
AU  - Crovetto G
FAU - Vilchez, J L
AU  - Vilchez JL
LA  - eng
PT  - Journal Article
PT  - Validation Study
PL  - England
TA  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess
JT  - Food additives & contaminants. Part A, Chemistry, analysis, control, exposure & 
      risk assessment
JID - 101485040
RN  - 0 (Pesticide Residues)
SB  - IM
MH  - Analytic Sample Preparation Methods
MH  - Calibration
MH  - Chromatography, High Pressure Liquid
MH  - Europe
MH  - *Food Contamination
MH  - Food Inspection/*methods/*standards
MH  - Fruit/*chemistry
MH  - Limit of Detection
MH  - Pesticide Residues/*analysis/chemistry
MH  - Reproducibility of Results
MH  - Spectrometry, Mass, Electrospray Ionization
MH  - Tandem Mass Spectrometry
MH  - Vegetables/*chemistry
EDAT- 2010/08/24 06:00
MHDA- 2011/01/29 06:00
CRDT- 2010/08/24 06:00
PHST- 2010/08/24 06:00 [entrez]
PHST- 2010/08/24 06:00 [pubmed]
PHST- 2011/01/29 06:00 [medline]
AID - 926036115 [pii]
AID - 10.1080/19440049.2010.506602 [doi]
PST - ppublish
SO  - Food Addit Contam Part A Chem Anal Control Expo Risk Assess. 2010 
      Nov;27(11):1532-44. doi: 10.1080/19440049.2010.506602.