PMID- 20732046
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20190918
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 5
IP  - 5-6
DP  - 1991
TI  - Measurement of inflammatory mediators produced by human keratinocytes in vitro: A 
      predictive assessment of cutaneous irritation.
PG  - 407-10
AB  - Two inflammatory mediators, interleukin 1alpha (IL1alpha) and prostaglandin E(2) 
      (PGE(2)), were determined by radioimmunoassay in both intracellular and 
      extracellular compartments from normal human keratinocytes in high-density 
      culture. Cells were subjected to different inflammatory stimuli: UVB irradiation 
      and detergent injury by sodium dodecyl sulphate (SDS). UVB irradiation produced 
      intracellular IL1alpha synthesis, and its dose-dependent release in the medium. 
      Similar results were obtained with SDS. The SDS stimulus was time dependent, and 
      the following mechanisms were observed: (i) increased production of intracellular 
      inflammatory mediators; and (ii) progressive release of these mediators in the 
      medium. With SDS, production and release of PGE(2) peaked below the lethal doses 
      (assessed by the neutral red bioassay). Therefore, the measurement of IL1alpha 
      and PGE(2), both intra- and extracellularly may be a useful in vitro method for 
      assessing the irritative potential of topically applied agents.
FAU - Cohen, C
AU  - Cohen C
AD  - L'Oreal Research Laboratories, Aulnay sous Bois, France.
FAU - Dossou, G
AU  - Dossou G
FAU - Rougier, A
AU  - Rougier A
FAU - Roguet, R
AU  - Roguet R
LA  - eng
PT  - Journal Article
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
EDAT- 1991/01/01 00:00
MHDA- 1991/01/01 00:01
CRDT- 2010/08/25 06:00
PHST- 2010/08/25 06:00 [entrez]
PHST- 1991/01/01 00:00 [pubmed]
PHST- 1991/01/01 00:01 [medline]
AID - 0887-2333(91)90062-I [pii]
AID - 10.1016/0887-2333(91)90062-i [doi]
PST - ppublish
SO  - Toxicol In Vitro. 1991;5(5-6):407-10. doi: 10.1016/0887-2333(91)90062-i.