PMID- 20732205
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20121002
LR  - 20190918
IS  - 0887-2333 (Print)
IS  - 0887-2333 (Linking)
VI  - 7
IP  - 4
DP  - 1993 Jul
TI  - Local lymph node assay: study of the in vitro proliferation and control of the 
      specificity of the response by FACScan analysis.
PG  - 299-303
AB  - The murine local lymph node assay (LLNA) has been proposed as a screening 
      procedure to identify contact allergens (Kimber, Hilton and Weisenberger, Contact 
      Dermatitis 1989, 21, 215; Kimber and Weisenberger, Archives of Toxicology 1989, 
      63, 274). In some cases irritants have given rise to proliferative responses and 
      it is of interest to investigate whether these responses differ in the type of 
      cells involved. We have studied the proliferative response in vitro to topically 
      applied sodium lauryl sulphate (SLS) and 2,4-dinitrochlorobenzene (DNCB). The 
      test chemical or vehicle alone was applied for 3 consecutive days to the dorsum 
      of both ears of Balb/c strain mice at three different concentrations, the highest 
      concentration being the maximum non-irritating concentration (MNIC). Cell 
      cultures were made 72 hr after the final exposure: draining auricular lymph nodes 
      were excised and a suspension of lymph node cells (LNC) was prepared. Cellularity 
      (total number of LNC/animal) and proliferative activity were assessed; 
      proliferation was measured by culture of LNC for 24 hr with [(3)H]thymidine. LNC 
      were also studied by FACScan analysis: cells were incubated with fluorescein 
      isothiocyanate (FITC)-labelled monoclonal antibodies against the T-cell marker 
      CD3 and the activated T-cell marker CD25 (IL2 receptor). In the case of DNCB 
      dose-response curves were obtained for both cellularity and proliferative 
      response in comparison with the controls: there was a strong increase in both 
      parameters for the MNIC.SLS, a non-sensitizing skin irritant, induced a much 
      lower response, slightly increased at the MNIC in comparison with the controls. 
      By FACScan analysis we measured the rates of CD3- and CD25-positive cells in the 
      LNC. No significant difference was obtained for SLS in comparison with the 
      controls. In the case of DNCB, there was a significant increase in CD3-positive 
      cells and a large increase in CD25-positive cells in comparison with the controls 
      and SLS. These parameters could be of great interest to help distinguish between 
      contact sensitizers and irritants. We are presently investigating other irritants 
      and sensitizers.
FAU - de Silva, O
AU  - de Silva O
AD  - Laboratoires de Recherche Fondamentale de L'Oreal, 1 avenue Eugene Schueller, 
      93600 Aulnay-Sous-Bois, France.
FAU - Perez, M J
AU  - Perez MJ
FAU - Pineau, N
AU  - Pineau N
FAU - Rougier, A
AU  - Rougier A
FAU - Dossou, K G
AU  - Dossou KG
LA  - eng
PT  - Journal Article
PL  - England
TA  - Toxicol In Vitro
JT  - Toxicology in vitro : an international journal published in association with 
      BIBRA
JID - 8712158
EDAT- 1993/07/01 00:00
MHDA- 1993/07/01 00:01
CRDT- 2010/08/25 06:00
PHST- 2010/08/25 06:00 [entrez]
PHST- 1993/07/01 00:00 [pubmed]
PHST- 1993/07/01 00:01 [medline]
AID - 0887-2333(93)90018-Z [pii]
AID - 10.1016/0887-2333(93)90018-z [doi]
PST - ppublish
SO  - Toxicol In Vitro. 1993 Jul;7(4):299-303. doi: 10.1016/0887-2333(93)90018-z.