PMID- 20732309
OWN - NLM
STAT- MEDLINE
DCOM- 20110118
LR  - 20131121
IS  - 1872-6240 (Electronic)
IS  - 0006-8993 (Linking)
VI  - 1358
DP  - 2010 Oct 28
TI  - Protective effect of 3-butyl-6-bromo-1(3H)-isobenzofuranone on hydrogen 
      peroxide-induced damage in PC12 cells.
PG  - 239-47
LID - 10.1016/j.brainres.2010.08.043 [doi]
AB  - Oxidative stress, an imbalance toward the prooxidant side of the 
      prooxidant/antioxidant homeostasis, occurs in several brain neurodegenerative 
      disorders. The protective effect of 3-butyl-6-bromo-1(3H)-isobenzofuranone 
      (Br-NBP), a derivative compound of l-3-n-butylphthalide (NBP), on the hydrogen 
      peroxide(H(2)O(2))- induced oxidative damage was investigated in PC12 cells. 
      Following exposure of the cells to H(2)O(2), there was a significant reduction in 
      cell survival,activities of glutathione peroxidase (GSH-px) and mitochondria 
      membrane potential(MMP), in contrast, the increased levels in the leakage of 
      lactate dehydrogenase (LDH), malondialdehyde (MDA) contents, nitric oxide (NO) 
      productions, intracellular reactive oxygen species (ROS), intracellular Ca(2+) 
      concentration ([Ca(2+)](i)), as well as cell apoptosis were observed. However, 
      pretreatment of cells with Br-NBP prior to H(2)O(2) exposure attenuated all the 
      changes mentioned above in a concentration-dependent manner except no effect on 
      activities of GSH-px. Br-NBP exhibited the protective effect against 
      H(2)O(2)-induced cytotoxicity in PC12 cells, suggesting that the compound may be 
      a potential therapeutic agent for diseases induced by oxidative damage.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Gao, Yuan
AU  - Gao Y
AD  - Department of Clinical Pharmacology, School of Medicine, Zhengzhou University, 
      Zhengzhou, Henan 450052, PR China.
FAU - Zhang, Hong-Wei
AU  - Zhang HW
FAU - Qiao, Hai-Ling
AU  - Qiao HL
FAU - Wang, Wei
AU  - Wang W
FAU - Chang, Jun-Biao
AU  - Chang JB
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100821
PL  - Netherlands
TA  - Brain Res
JT  - Brain research
JID - 0045503
RN  - 0 (Benzofurans)
RN  - 0 (Neuroprotective Agents)
RN  - 0 (Oxidants)
RN  - 0 (Reactive Oxygen Species)
RN  - 4Y8F71G49Q (Malondialdehyde)
RN  - 822Q956KGM (3-n-butylphthalide)
RN  - BBX060AN9V (Hydrogen Peroxide)
RN  - EC 1.1.1.27 (L-Lactate Dehydrogenase)
RN  - EC 1.11.1.9 (Glutathione Peroxidase)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Apoptosis/drug effects
MH  - Benzofurans/*pharmacology
MH  - Calcium/metabolism
MH  - Cell Survival/drug effects
MH  - Drug Interactions
MH  - Glutathione Peroxidase/metabolism
MH  - Hydrogen Peroxide/*toxicity
MH  - L-Lactate Dehydrogenase/metabolism
MH  - Malondialdehyde/metabolism
MH  - Membrane Potential, Mitochondrial/drug effects
MH  - Neuroprotective Agents/*pharmacology
MH  - Oxidants/*toxicity
MH  - Oxidative Stress/*drug effects
MH  - PC12 Cells/drug effects
MH  - Rats
MH  - Reactive Oxygen Species/metabolism
EDAT- 2010/08/25 06:00
MHDA- 2011/01/19 06:00
CRDT- 2010/08/25 06:00
PHST- 2010/05/20 00:00 [received]
PHST- 2010/08/13 00:00 [revised]
PHST- 2010/08/13 00:00 [accepted]
PHST- 2010/08/25 06:00 [entrez]
PHST- 2010/08/25 06:00 [pubmed]
PHST- 2011/01/19 06:00 [medline]
AID - S0006-8993(10)01825-1 [pii]
AID - 10.1016/j.brainres.2010.08.043 [doi]
PST - ppublish
SO  - Brain Res. 2010 Oct 28;1358:239-47. doi: 10.1016/j.brainres.2010.08.043. Epub 
      2010 Aug 21.