PMID- 20732430
OWN - NLM
STAT- MEDLINE
DCOM- 20110203
LR  - 20101012
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 29
IP  - 6
DP  - 2010 Dec
TI  - The iron-cofactored superoxide dismutase of Edwardsiella tarda inhibits 
      macrophage-mediated innate immune response.
PG  - 972-8
LID - 10.1016/j.fsi.2010.08.004 [doi]
AB  - Edwardsiella tarda is a Gram-negative bacterium that can infect a wide variety of 
      marine and freshwater fish and cause severe economic losses worldwide. With an 
      aim to elucidate the virulence mechanism of E. tarda, we in this study cloned and 
      analyzed the function of an iron-cofactored superoxide dismutase (FeSOD) from a 
      pathogenic E. tarda strain TX01 isolated from diseased fish. FeSOD is 192-residue 
      in length and contains domain structures that are conserved among iron/manganese 
      superoxide dismutases. Recombinant FeSOD purified from Escherichia coli exhibits 
      apparent superoxide dismutase activity. Quantitative real-time RT-PCR analysis 
      indicated that FeSOD expression is significantly upregulated immediately 
      following TX01 infection of Japanese flounder (Paralichthys olivaceus) head 
      kidney (HK) macrophages and cultured FG cells. Compared to the wild type strain 
      TX01, the FeSOD mutant strain TXSod is (i) more sensitive to H(2)O(2)-induced 
      oxidative damage, (ii) less resistant against serum- and macrophage-mediated 
      bacterial killing, (iii) significantly weakened in the ability to invade into FG 
      cells and to disseminate in fish blood and liver, and (iv) deficient in blocking 
      macrophage respiratory burst activity and production of reactive oxygen species. 
      Furthermore, HK macrophages infected by TXSod exhibits significantly increased 
      expression of inflammatory cytokines compared to macrophages infected by TX01. 
      Taken together, these results indicate that FeSOD is a virulence factor that 
      plays an important role in the pathogenicity of E. tarda by inhibiting 
      macrophage-mediated innate immune response.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Cheng, Shuang
AU  - Cheng S
AD  - Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese 
      Academy of Sciences, Qingdao, PR China.
FAU - Zhang, Min
AU  - Zhang M
FAU - Sun, Li
AU  - Sun L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100821
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (DNA, Bacterial)
RN  - 0 (Recombinant Proteins)
RN  - EC 1.15.1.1 (Superoxide Dismutase)
SB  - IM
MH  - Animals
MH  - Cloning, Molecular
MH  - DNA, Bacterial/chemistry/genetics
MH  - Edwardsiella tarda/*enzymology/genetics/immunology/pathogenicity
MH  - Enterobacteriaceae Infections/enzymology/microbiology/*veterinary
MH  - Fish Diseases/immunology/*microbiology
MH  - *Flounder
MH  - Immunity, Innate/immunology
MH  - Macrophages/*immunology
MH  - Mutagenesis, Site-Directed/veterinary
MH  - Polymerase Chain Reaction/veterinary
MH  - Recombinant Proteins/genetics/immunology
MH  - Sequence Analysis, DNA
MH  - Superoxide Dismutase/genetics/*immunology
MH  - Virulence/immunology
EDAT- 2010/08/25 06:00
MHDA- 2011/02/04 06:00
CRDT- 2010/08/25 06:00
PHST- 2010/07/11 00:00 [received]
PHST- 2010/08/10 00:00 [revised]
PHST- 2010/08/10 00:00 [accepted]
PHST- 2010/08/25 06:00 [entrez]
PHST- 2010/08/25 06:00 [pubmed]
PHST- 2011/02/04 06:00 [medline]
AID - S1050-4648(10)00257-3 [pii]
AID - 10.1016/j.fsi.2010.08.004 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2010 Dec;29(6):972-8. doi: 10.1016/j.fsi.2010.08.004. 
      Epub 2010 Aug 21.