PMID- 20738914
OWN - NLM
STAT- PubMed-not-MEDLINE
DCOM- 20130101
LR  - 20100826
IS  - 1999-6187 (Electronic)
IS  - 1009-3419 (Linking)
VI  - 11
IP  - 5
DP  - 2008 Oct 20
TI  - [The expression of ABCG4, V-ATPase and clinic significance of their correlation 
      with NSCLC.].
PG  - 691-5
LID - 10.3779/j.issn.1009-3419.2008.05.023 [doi]
AB  - BACKGROUND: It has been proven that the multiple drug resistance is main reason 
      for failure of chemotherapy in lung cancers and ABC transporter play a main role 
      for chemoresistance in mediating drug efflux. So searching for new drug resistant 
      protein of the ABC family and elucidating its resistant mechanism is very 
      important. ABCG4 is one of ABC family and is expected to be candidate drug 
      resistant protein; and the drug resistance probably correlated with pH value 
      around cancer cell, while, V-ATPase play key role in modulating the pH. So our 
      aim is to investigate the expressions of ABCG4, V-ATPase proteins in non small 
      cell lung cancer (NSCLC), and analyze relationship of ABCG4, V-ATPase protein 
      expressional rate in these cancers with the cancers' pathological grade and TNM 
      stages. METHODS: To detect the expression rates of ABCG4, V-ATPase protein in 
      NSCLC with immunohistochemical method and immuno- fluorescent method, and to 
      observe the location, the collocation of the proteins under light microscope and 
      confocal laser scanning microscope; the differences of the protein expression and 
      their correlations were analyzed by statistics. RESULTS: ABCG4 protein was high 
      expressed in squamous cell lung cancer, lung adenocarcinoma respectively, and 
      between the two kinds of the cancers there was a significant difference (P 
      =0.001) for their comparison; there were significant differences between 
      pathological grade II and II-III of squamous cell lung cancer, between different 
      differentiated lung adenocarcinoma. V-ATPase protein were also high expressed in 
      these two kinds of cancers, and there was significant difference for their 
      comparison; there were significant differences between pathological grade II and 
      II-III of squamous cell lung cancer, between different differentiated lung 
      adenocarcinoma; there were no significant differences among the squamous cell 
      lung cancer and lung adenocarcinoma for TNM stages respectively. The P values of 
      correlationship test of positive intensity between ABCG4 and V-ATPase expression 
      in the total squamous cell lung cancer and lung adenocarcinoma, in the 
      pathological grade II and II-III of the squamous cell lung cancer, in the 
      moderately differentiated lung adenocarcinoma were all 0.000, while the 
      correlation coefficients were 0.771, 0.765, 0.714, 0.777, 0.865 respectively; 
      however, the P values of correlationship test of between ABCG4 and V-ATPase 
      expression in the low differentiated lung adenocarcinoma was 0.048, and the 
      correlation coefficient was 0.35. The ABCG4 and V-ATPase positive expression was 
      mainly located in the plasma membrane and cytoplasm, and there was collocation 
      among the ABCG4 and V-ATPase proteins. CONCLUSIONS: The ABCG4 and V-ATPase 
      proteins are highly expressed in the NSCLC, and the expression rates of ABCG4, 
      V-ATPase proteins are correlated with the cancers' pathological grades and TNM 
      stage; and there are all having correlationship between ABCG4 and V-ATPase 
      proteins in the squamous cell lung cancer, in the lung adenocarcinoma, in all 
      their pathological grades.
FAU - Zhang, Zhipei
AU  - Zhang Z
AD  - Department of Thoracic Surgery, Tangdu Hospital, Fourth Military Medical 
      University, Xi'an 710038, China.
FAU - Li, Xiaofei
AU  - Li X
FAU - Wang, Xiaoping
AU  - Wang X
FAU - Zhou, Yong'an
AU  - Zhou Y
FAU - Xu, Hong
AU  - Xu H
FAU - Wang, Jian
AU  - Wang J
FAU - Huang, Lijun
AU  - Huang L
FAU - Tian, Yutu
AU  - Tian Y
FAU - Cheng, Qingshu
AU  - Cheng Q
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Zhongguo Fei Ai Za Zhi
JT  - Zhongguo fei ai za zhi = Chinese journal of lung cancer
JID - 101126433
EDAT- 2008/10/20 00:00
MHDA- 2008/10/20 00:01
CRDT- 2010/08/27 06:00
PHST- 2010/08/27 06:00 [entrez]
PHST- 2008/10/20 00:00 [pubmed]
PHST- 2008/10/20 00:01 [medline]
AID - 10.3779/j.issn.1009-3419.2008.05.023 [doi]
PST - ppublish
SO  - Zhongguo Fei Ai Za Zhi. 2008 Oct 20;11(5):691-5. doi: 
      10.3779/j.issn.1009-3419.2008.05.023.