PMID- 20739052
OWN - NLM
STAT- MEDLINE
DCOM- 20110408
LR  - 20101207
IS  - 1879-3231 (Electronic)
IS  - 0093-691X (Linking)
VI  - 75
IP  - 1
DP  - 2011 Jan 1
TI  - Molecular delineation of the Y-borne Sry gene in the Formosan pangolin (Manis 
      pentadactyla pentadactyla) and its phylogenetic implications for Pholidota in 
      extant mammals.
PG  - 55-64
LID - 10.1016/j.theriogenology.2010.07.010 [doi]
AB  - The systematic status of Pholidota has been a matter of debate, particularly 
      regarding the apparent inconsistency between morphological and molecular studies. 
      The Sry gene, a master regulator of male sex determination in eutherian mammals, 
      has not yet been used for phylogenetic analyses of extant mammals. The objective 
      of the present study was to clone and characterize the complete gene (1300 base 
      pairs; bp) and amino acid sequences (229 residues) of Sry from the Formosan 
      pangolin (Manis pentadactyla pentadactyla), a member of Pholidota. The Sry amino 
      acid identity between pangolin and other reported species ranged from 42.5% 
      (mouse, Mus musculus) to 84.1% (European hare, Lepus europaeus). Sequence 
      conservation was primarily in the high motility group (HMG) box (234 bp), whereas 
      homology outside the HMG box was low. The cloned Sry was mapped to the pangolin Y 
      chromosome by fluorescence in situ hybridization (FISH); this was confirmed to be 
      the first Y-borne molecular marker identified in Pholidota. Based on Bayesian 
      phylogenetic analysis for Sry HMG sequences from 36 representative taxa, 
      including the Formosan pangolin, Pholidota was more closely related to Carnivora 
      than to Xenarthra, consistent with the emerging molecular tree inferred from 
      markers not located on the Y chromosome. In conclusion, this study characterized 
      the gene structure of Sry of the Formosan pangolin and provided insights into the 
      phylogenetic position of Pholidota.
CI  - Copyright (c) 2011 Elsevier Inc. All rights reserved.
FAU - Yu, Hon-Tsen
AU  - Yu HT
AD  - Institute of Zoology, Department of Life Science, National Taiwan University, 
      Taipei, Taiwan.
FAU - Ma, Gwo-Chin
AU  - Ma GC
FAU - Lee, Dong-Jay
AU  - Lee DJ
FAU - Chin, Shih-Chien
AU  - Chin SC
FAU - Tsao, Hsien-Shao
AU  - Tsao HS
FAU - Wu, Sheng-Hai
AU  - Wu SH
FAU - Shih, Shu-Yi
AU  - Shih SY
FAU - Chen, Ming
AU  - Chen M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100823
PL  - United States
TA  - Theriogenology
JT  - Theriogenology
JID - 0421510
SB  - IM
MH  - Amino Acid Sequence
MH  - Animals
MH  - Base Sequence
MH  - Bayes Theorem
MH  - Cloning, Molecular
MH  - Conserved Sequence
MH  - *Genes, sry
MH  - In Situ Hybridization, Fluorescence
MH  - Mammals/classification/*genetics
MH  - Molecular Sequence Data
MH  - *Phylogeny
MH  - Sequence Analysis, Protein
MH  - Y Chromosome/chemistry
EDAT- 2010/08/27 06:00
MHDA- 2011/04/09 06:00
CRDT- 2010/08/27 06:00
PHST- 2009/12/14 00:00 [received]
PHST- 2010/07/16 00:00 [revised]
PHST- 2010/07/18 00:00 [accepted]
PHST- 2010/08/27 06:00 [entrez]
PHST- 2010/08/27 06:00 [pubmed]
PHST- 2011/04/09 06:00 [medline]
AID - S0093-691X(10)00387-0 [pii]
AID - 10.1016/j.theriogenology.2010.07.010 [doi]
PST - ppublish
SO  - Theriogenology. 2011 Jan 1;75(1):55-64. doi: 
      10.1016/j.theriogenology.2010.07.010. Epub 2010 Aug 23.