PMID- 20795757
OWN - NLM
STAT- MEDLINE
DCOM- 20110606
LR  - 20180629
IS  - 1477-2566 (Electronic)
IS  - 1465-3249 (Linking)
VI  - 13
IP  - 3
DP  - 2011 Mar
TI  - Adipose tissue-derived stem cells show both immunogenic and immunosuppressive 
      properties after chondrogenic differentiation.
PG  - 310-7
LID - 10.3109/14653249.2010.504769 [doi]
AB  - BACKGROUND AIMS: The chondrogenic differentiation potential of mesenchymal 
      stromal cells (MSC), as well as their immunosuppressive properties, have been 
      studied extensively. So far, only a few studies have addressed the question of 
      whether MSC still retain their immunosuppressive qualities after 
      transdifferentiation. In particular, the expression of immunogenic markers, such 
      as human leukocyte antigen (HLA)-DR, after differentiation has never been 
      investigated. METHODS: Chondrogenic transdifferentiation was induced in human 
      adipose tissue-derived stem cell (ADSC) pellet cultures derived from 10 different 
      patients, using 10 ng/mL transforming growth factor (TGF)-beta3. Samples were 
      harvested over a time-course of 28 days and analyzed by immunohistochemistry and 
      reverse transcription (RT)-polymerase chain reaction (PCR). The cytokine levels 
      in the supernatants of the samples were measured semi-quantitatively by dot-blots 
      and quantitatively by enzyme-linked immunosorbant assays (ELISA). RESULTS: 
      Undifferentiated ADSC were negative for chondrogenic markers, as well as HLA-ABC 
      and HLA-DR epitopes in immunofluorescence. In contrast, TGF-beta3-induced pellet 
      cultures showed both expression of chondrogenic differentiation markers, such as 
      transcription factor 9 (Sox 9), collagen type IIa and aggrecan, and an 
      up-regulation of HLA-DR, beginning at day 7 after induction. Interferon-gamma (INF-gamma) 
      is known to up-regulate HLA-DR. Therefore we measured INF-gamma levels in the 
      supernatants of TGF-beta3-induced pellets and, indeed, INF-gamma was up-regulated during 
      chondrogenesis in ADSC pellet cultures. However, both undifferentiated and 
      TGF-beta3-induced ADSC also showed expression of immunosuppressive HLA-G and 
      interleukin (IL)-10 up-regulation. CONCLUSIONS: These results suggest that the 
      immunogenicity of adult stem cell-derived tissue should be tested in animal 
      models before clinical trials for allogeneic engineered tissue are considered.
FAU - Technau, Antje
AU  - Technau A
AD  - Department of Otorhinolaryngology, Plastic, Aesthetic and Reconstructive Head and 
      Neck Surgery, University of Wuerzburg, Wuerzburg, Germany.
FAU - Froelich, Katrin
AU  - Froelich K
FAU - Hagen, Rudolf
AU  - Hagen R
FAU - Kleinsasser, Norbert
AU  - Kleinsasser N
LA  - eng
PT  - Journal Article
DEP - 20100826
PL  - England
TA  - Cytotherapy
JT  - Cytotherapy
JID - 100895309
RN  - 0 (Biomarkers)
RN  - 0 (Epitopes)
RN  - 0 (HLA-DR Antigens)
RN  - 130068-27-8 (Interleukin-10)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Adipose Tissue/*cytology
MH  - Biomarkers/metabolism
MH  - Cell Differentiation/*immunology
MH  - Cells, Cultured
MH  - Chondrogenesis/*immunology
MH  - Epitopes/genetics/immunology
MH  - HLA-DR Antigens/immunology
MH  - Humans
MH  - Immune Tolerance/*immunology
MH  - Immunoassay
MH  - Interferon-gamma/metabolism
MH  - Interleukin-10/metabolism
MH  - Stem Cells/cytology/*immunology
MH  - Subcellular Fractions/metabolism
MH  - Up-Regulation
EDAT- 2010/08/28 06:00
MHDA- 2011/06/07 06:00
CRDT- 2010/08/28 06:00
PHST- 2010/08/28 06:00 [entrez]
PHST- 2010/08/28 06:00 [pubmed]
PHST- 2011/06/07 06:00 [medline]
AID - S1465-3249(11)70507-2 [pii]
AID - 10.3109/14653249.2010.504769 [doi]
PST - ppublish
SO  - Cytotherapy. 2011 Mar;13(3):310-7. doi: 10.3109/14653249.2010.504769. Epub 2010 
      Aug 26.