PMID- 20798376
OWN - NLM
STAT- MEDLINE
DCOM- 20101220
LR  - 20211020
IS  - 1524-4636 (Electronic)
IS  - 1079-5642 (Print)
IS  - 1079-5642 (Linking)
VI  - 30
IP  - 11
DP  - 2010 Nov
TI  - ATP-binding cassette transporter G1 and high-density lipoprotein promote 
      endothelial NO synthesis through a decrease in the interaction of caveolin-1 and 
      endothelial NO synthase.
PG  - 2219-25
LID - 10.1161/ATVBAHA.110.213215 [doi]
AB  - OBJECTIVE: To investigate whether cholesterol efflux to high-density lipoprotein 
      (HDL) via ATP-binding cassette transporter G1 (ABCG1) modulates the interaction 
      of caveolin (Cav) 1 and endothelial NO synthase (eNOS). METHODS AND RESULTS: 
      ABCG1 promotes cholesterol and 7-oxysterol efflux from endothelial cells (ECs) to 
      HDL. It was previously reported that ABCG1 protects against dietary 
      cholesterol-induced endothelial dysfunction by promoting the efflux of 
      7-oxysterols to HDL. Increased cholesterol loading in ECs is known to cause an 
      inhibitory interaction between Cav-1 and eNOS and impaired NO release. In human 
      aortic ECs, free cholesterol loading promoted the interaction of Cav-1 with eNOS, 
      reducing eNOS activity. These effects of cholesterol loading were reversed by HDL 
      in an ABCG1-dependent manner. HDL also reversed the inhibition of eNOS by 
      cholesterol loading in murine lung ECs, but this effect of HDL was abolished in 
      Cav-1-deficient murine lung ECs. Increased interaction of Cav-1 with eNOS was 
      also detected in aortic homogenates of high-cholesterol diet-fed Abcg1(-/-) mice, 
      paralleling a decrease in eNOS activity and impaired endothelial function. 
      CONCLUSIONS: The promotion of cholesterol efflux via ABCG1 results in a reduced 
      inhibitory interaction of eNOS with Cav-1.
FAU - Terasaka, Naoki
AU  - Terasaka N
AD  - Division of Molecular Medicine, Department of Medicine, Columbia University, 630 
      W 168 St, New York, NY 10032, USA.
FAU - Westerterp, Marit
AU  - Westerterp M
FAU - Koetsveld, Joris
AU  - Koetsveld J
FAU - Fernandez-Hernando, Carlos
AU  - Fernandez-Hernando C
FAU - Yvan-Charvet, Laurent
AU  - Yvan-Charvet L
FAU - Wang, Nan
AU  - Wang N
FAU - Sessa, William C
AU  - Sessa WC
FAU - Tall, Alan R
AU  - Tall AR
LA  - eng
GR  - R01 HL061371/HL/NHLBI NIH HHS/United States
GR  - R37 HL061371/HL/NHLBI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100826
PL  - United States
TA  - Arterioscler Thromb Vasc Biol
JT  - Arteriosclerosis, thrombosis, and vascular biology
JID - 9505803
RN  - 0 (ABCG1 protein, human)
RN  - 0 (ATP Binding Cassette Transporter, Subfamily G, Member 1)
RN  - 0 (ATP-Binding Cassette Transporters)
RN  - 0 (Caveolin 1)
RN  - 0 (Cholesterol, HDL)
RN  - 31C4KY9ESH (Nitric Oxide)
RN  - EC 1.14.13.39 (NOS3 protein, human)
RN  - EC 1.14.13.39 (Nitric Oxide Synthase Type III)
SB  - IM
MH  - ATP Binding Cassette Transporter, Subfamily G, Member 1
MH  - ATP-Binding Cassette Transporters/*metabolism
MH  - Animals
MH  - Caveolin 1/*metabolism
MH  - Cholesterol, HDL/*metabolism
MH  - Endothelial Cells
MH  - Humans
MH  - Mice
MH  - Nitric Oxide/*biosynthesis
MH  - Nitric Oxide Synthase Type III/*metabolism
PMC - PMC4790108
MID - NIHMS759718
EDAT- 2010/08/28 06:00
MHDA- 2010/12/21 06:00
PMCR- 2016/03/14
CRDT- 2010/08/28 06:00
PHST- 2010/08/28 06:00 [entrez]
PHST- 2010/08/28 06:00 [pubmed]
PHST- 2010/12/21 06:00 [medline]
PHST- 2016/03/14 00:00 [pmc-release]
AID - ATVBAHA.110.213215 [pii]
AID - 10.1161/ATVBAHA.110.213215 [doi]
PST - ppublish
SO  - Arterioscler Thromb Vasc Biol. 2010 Nov;30(11):2219-25. doi: 
      10.1161/ATVBAHA.110.213215. Epub 2010 Aug 26.