PMID- 20798552
OWN - NLM
STAT- MEDLINE
DCOM- 20110614
LR  - 20161125
IS  - 1661-7819 (Electronic)
IS  - 1661-7800 (Linking)
VI  - 99
IP  - 2
DP  - 2011
TI  - Lipopolysaccharide-induced expression of Th1/Th2 cytokines in whole neonatal cord 
      and adult blood: role of nuclear factor-kappa B and p38 MAPK.
PG  - 140-5
LID - 10.1159/000313967 [doi]
AB  - BACKGROUND: Sepsis continues to be a leading cause of morbidity and mortality in 
      newborns. OBJECTIVE: As both nuclear factor-kappa B (NF-kappaB) and p38 
      mitogen-activated protein kinase (MAPK) appear to be critical mediators in 
      inflammatory response, we studied the effects of lipopolysaccharide (LPS) on 
      expression and function of NF-kappaB and p38 MAPK in whole neonatal cord and adult 
      blood. METHODS: Th1/Th2 cytokine concentrations and phosphorylation of NF-kappaB and 
      p38 MAPK were determined by flow-cytometric analysis. RESULTS: Tumor necrosis 
      factor-alpha (TNF-alpha), IL-6, and IL-10 concentrations were significantly elevated 
      in supernatants of neonatal and adult blood after LPS stimulation for 4 h. IFN-gamma, 
      IL-4, and IL-2 showed no significant alterations. Furthermore, TNF-alpha 
      concentrations were significantly higher in adult compared to neonatal blood 
      after LPS stimulation. Stimulation with LPS resulted in significantly decreased 
      activation of p38 MAPK in neonatal blood, whereas NF-kappaB showed no difference. 
      Following inhibition of p38 MAPK with the specific inhibitor SB-202190, levels of 
      TNF-alpha and IL-6 significantly decreased in neonatal and adult blood, whereas 
      pharmacological inhibition of NF-kappaB with SC-514 showed no significant effect on 
      cytokine expression. CONCLUSIONS: We conclude that p38 MAPK phosphorylation is 
      crucially involved in LPS activation and could explain the differences in early 
      cytokine response between neonatal and adult blood.
CI  - Copyright (c) 2010 S. Karger AG, Basel.
FAU - Koch, Lutz
AU  - Koch L
AD  - Department of Neonatology, University of Heidelberg, Medical School, Heidelberg, 
      Germany. lutz.koch@med.uni-heidelberg.de
FAU - Fritzsching, Benedikt
AU  - Fritzsching B
FAU - Frommhold, David
AU  - Frommhold D
FAU - Poeschl, Johannes
AU  - Poeschl J
LA  - eng
PT  - Journal Article
DEP - 20100827
PL  - Switzerland
TA  - Neonatology
JT  - Neonatology
JID - 101286577
RN  - 0 (Cytokines)
RN  - 0 (Enzyme Inhibitors)
RN  - 0 (Imidazoles)
RN  - 0 (Lipopolysaccharides)
RN  - 0 (NF-kappa B)
RN  - 0 (Pyridines)
RN  - 0 (SC 514)
RN  - 0 (Thiophenes)
RN  - EC 2.7.11.24 (p38 Mitogen-Activated Protein Kinases)
RN  - PVX798P8GI (4-(4-fluorophenyl)-2-(4-hydroxyphenyl)-5-(4-pyridyl)imidazole)
SB  - IM
MH  - Adult
MH  - Cytokines/*biosynthesis/blood
MH  - Enzyme Inhibitors/pharmacology
MH  - Fetal Blood/drug effects/enzymology/*immunology
MH  - Flow Cytometry
MH  - Humans
MH  - Imidazoles/pharmacology
MH  - Infant, Newborn
MH  - Lipopolysaccharides/*pharmacology
MH  - NF-kappa B/antagonists & inhibitors/biosynthesis/blood
MH  - Phosphorylation/drug effects
MH  - Pyridines/pharmacology
MH  - Signal Transduction
MH  - Th1 Cells/drug effects/enzymology/immunology/*metabolism
MH  - Th2 Cells/drug effects/enzymology/immunology/*metabolism
MH  - Thiophenes/pharmacology
MH  - p38 Mitogen-Activated Protein Kinases/antagonists & 
      inhibitors/*biosynthesis/blood
EDAT- 2010/08/28 06:00
MHDA- 2011/06/15 06:00
CRDT- 2010/08/28 06:00
PHST- 2010/01/22 00:00 [received]
PHST- 2010/04/15 00:00 [accepted]
PHST- 2010/08/28 06:00 [entrez]
PHST- 2010/08/28 06:00 [pubmed]
PHST- 2011/06/15 06:00 [medline]
AID - 000313967 [pii]
AID - 10.1159/000313967 [doi]
PST - ppublish
SO  - Neonatology. 2011;99(2):140-5. doi: 10.1159/000313967. Epub 2010 Aug 27.