PMID- 20800644
OWN - NLM
STAT- MEDLINE
DCOM- 20110111
LR  - 20211020
IS  - 1873-2399 (Electronic)
IS  - 0301-472X (Print)
IS  - 0301-472X (Linking)
VI  - 38
IP  - 12
DP  - 2010 Dec
TI  - Evidence of mobilization of pluripotent stem cells into peripheral blood of 
      patients with myocardial ischemia.
PG  - 1131-1142.e1
LID - 10.1016/j.exphem.2010.08.003 [doi]
AB  - OBJECTIVE: The ischemic myocardium releases multiple chemotactic factors 
      responsible for the mobilization and recruitment of bone marrow-derived cells to 
      injured myocardium. However, the mobilization of primitive pluripotent stem cells 
      (PSCs) enriched in very small embryonic-like stem cells (VSELs) in various 
      cardiac ischemic scenarios is not well understood. MATERIALS AND METHODS: 
      Fifty-four ischemic heart disease patients, including subjects with stable 
      angina, non-ST elevation myocardial infarction, and ST elevation myocardial 
      infarction (STEMI) and 12 matched controls were enrolled. The absolute numbers of 
      circulating stem/primitive cells in samples of peripheral blood (PB) were 
      quantitated by ImageStream analysis and conventional flow cytometry. Gene 
      expression of PSC (Oct-4 and Nanog), early cardiomyocyte (Nkx-2.5 and GATA-4), 
      and endothelial (von Willebrand factor) markers was analyzed by real-time 
      polymerase chain reaction. RESULTS: The absolute numbers of PSCs, stem cell 
      populations enriched in VSELs, and hematopoietic stem cells present in PB were 
      significantly higher in STEMI patients at presentation and declined over time. 
      There was a corresponding increase in pluripotent, cardiac, and endothelial gene 
      expression in unfractionated PB cells and sorted PB-derived primitive CD34(+) 
      cells. The absolute numbers of circulating VSELs and hematopoietic stem cells in 
      STEMI correlated negatively with patient age. CONCLUSIONS: Myocardial ischemia 
      mobilizes primitive PSCs including pluripotent VSELs into the circulation. The 
      peak of mobilization occurs within 12 hours in patients presenting with STEMI, 
      which may represent a therapeutic window for future clinical applications. 
      Reduced stem cell mobilization with advancing age could explain, in part, the 
      observation that age is associated with poor prognosis in patients with 
      myocardial infarction.
CI  - Published by Elsevier Inc.
FAU - Abdel-Latif, Ahmed
AU  - Abdel-Latif A
AD  - Gill Heart Institute and Division of Cardiovascular Medicine, University of 
      Kentucky, 740 S. Limestone Street, Lexington, KY 40536-0200, USA. 
      abdel-latif@uky.edu
FAU - Zuba-Surma, Ewa K
AU  - Zuba-Surma EK
FAU - Ziada, Khaled M
AU  - Ziada KM
FAU - Kucia, Magdalena
AU  - Kucia M
FAU - Cohen, Donald A
AU  - Cohen DA
FAU - Kaplan, Alan M
AU  - Kaplan AM
FAU - Van Zant, Gary
AU  - Van Zant G
FAU - Selim, Samy
AU  - Selim S
FAU - Smyth, Susan S
AU  - Smyth SS
FAU - Ratajczak, Mariusz Z
AU  - Ratajczak MZ
LA  - eng
GR  - R01 DK074720-04/DK/NIDDK NIH HHS/United States
GR  - R01 DK074720/DK/NIDDK NIH HHS/United States
GR  - R01 HL078663/HL/NHLBI NIH HHS/United States
GR  - NIH R01 DK074720/DK/NIDDK NIH HHS/United States
GR  - R01 CA106281/CA/NCI NIH HHS/United States
GR  - R01 CA106281-05/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100826
PL  - Netherlands
TA  - Exp Hematol
JT  - Experimental hematology
JID - 0402313
RN  - 0 (Cytokines)
RN  - 0 (Octamer Transcription Factor-3)
RN  - 0 (POU5F1 protein, human)
RN  - 0 (Stage-Specific Embryonic Antigens)
RN  - 0 (stage-specific embryonic antigen-4)
SB  - IM
MH  - Adult
MH  - Age Factors
MH  - Aged
MH  - Cytokines/blood
MH  - Female
MH  - Flow Cytometry
MH  - *Hematopoietic Stem Cell Mobilization
MH  - Humans
MH  - Immunophenotyping
MH  - Male
MH  - Middle Aged
MH  - Myocardial Ischemia/*blood/therapy
MH  - Octamer Transcription Factor-3/analysis
MH  - Pluripotent Stem Cells/*physiology
MH  - Reverse Transcriptase Polymerase Chain Reaction
MH  - Stage-Specific Embryonic Antigens/analysis
PMC - PMC2992878
MID - NIHMS246525
EDAT- 2010/08/31 06:00
MHDA- 2011/01/12 06:00
PMCR- 2011/12/01
CRDT- 2010/08/31 06:00
PHST- 2010/03/22 00:00 [received]
PHST- 2010/07/08 00:00 [revised]
PHST- 2010/08/11 00:00 [accepted]
PHST- 2010/08/31 06:00 [entrez]
PHST- 2010/08/31 06:00 [pubmed]
PHST- 2011/01/12 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - S0301-472X(10)00367-X [pii]
AID - 10.1016/j.exphem.2010.08.003 [doi]
PST - ppublish
SO  - Exp Hematol. 2010 Dec;38(12):1131-1142.e1. doi: 10.1016/j.exphem.2010.08.003. 
      Epub 2010 Aug 26.