PMID- 20801009
OWN - NLM
STAT- MEDLINE
DCOM- 20101015
LR  - 20181201
IS  - 1618-0402 (Electronic)
IS  - 0940-9602 (Linking)
VI  - 192
IP  - 5
DP  - 2010 Sep 20
TI  - Bone marrow stromal cells as an inducer for cardiomyocyte differentiation from 
      mouse embryonic stem cells.
PG  - 314-21
LID - 10.1016/j.aanat.2010.07.001 [doi]
AB  - Bone marrow stromal cells (BMSCs) secrete soluble factors and display varied 
      cell-biological functions. To confirm the ability and efficiency of BMSCs to 
      induce embryonic stem cells (ESCs) into cardiomyocytes, mouse embryoid bodies 
      (EBs) were co-cultured with rat BMSCs. After about 10 days, areas of rhythmically 
      contracting cells in more solid aggregates became evident with bundle-like 
      structures formed along borders between EB outgrowth and BMSC layer. ESC-derived 
      cardiomyocytes exhibited sarcomeric striations when stained with troponin I (Trop 
      I), organized in separated bundles. Besides, the staining for connexin 43 was 
      detected in cell-cell junctions, which demonstrated that ESC-derived 
      cardiomyocytes were coupled by gap junction in culture. The related genes of 
      cardiomyocytes were found in these beating and no-beating EBs co-cultured with 
      BMSCs. In addition, an improved efficiency of cardiomyocyte differentiation from 
      ESC-BMSC co-culture was found in the serum-free medium: 5-fold up-regulation in 
      the number of beating area compared with the serum medium. Effective cardiac 
      differentiation was also recognized in transfer filter assay and in condition 
      medium obtained from BMSC culture. A clear increase in the expression of cardiac 
      genes and TropI protein confirmed further cardiac differentiation by BMP4 and 
      Retinoic Acid (RA) treatment. These results demonstrate that BMSCs can induce 
      cardiomyocyte differentiation from ESCs through soluble factors and enhance it 
      with BMP4 or RA treatment. Serum-free ESC-BMSC co-culture represents a defined in 
      vitro model for identifying the cardiomyocyte-inducing activity from BMSCs and, 
      in addition, a straightforward experimental system for assessing clinical 
      applications.
CI  - Copyright (c) 2010 Elsevier GmbH. All rights reserved.
FAU - Yue, Fengming
AU  - Yue F
AD  - Department of Histology and Embryology, Institute of Organ Transplants, 
      Reconstructive Medicine and Tissue Engineering, Shinshu University Graduate 
      School of Medicine, 3-1-1 Asahi, Matsumoto 390-8621, Nagano, Japan. 
      yueratjp@shinshu-u.ac.jp
FAU - Johkura, Kohei
AU  - Johkura K
FAU - Tomotsune, Daihachiro
AU  - Tomotsune D
FAU - Shirasawa, Sakiko
AU  - Shirasawa S
FAU - Yokoyama, Tadayuki
AU  - Yokoyama T
FAU - Nagai, Mika
AU  - Nagai M
FAU - Sasaki, Katsunori
AU  - Sasaki K
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100805
PL  - Germany
TA  - Ann Anat
JT  - Annals of anatomy = Anatomischer Anzeiger : official organ of the Anatomische 
      Gesellschaft
JID - 100963897
RN  - 0 (Bone Morphogenetic Protein 4)
RN  - 0 (Connexin 43)
RN  - 0 (Culture Media, Serum-Free)
SB  - IM
MH  - Animals
MH  - Bone Marrow Cells/cytology/metabolism/*physiology
MH  - Bone Morphogenetic Protein 4/pharmacology
MH  - Cell Differentiation/drug effects/physiology
MH  - Cells, Cultured
MH  - Coculture Techniques
MH  - Connexin 43/metabolism
MH  - Culture Media, Serum-Free/pharmacology
MH  - Embryonic Stem Cells/*cytology/drug effects/metabolism
MH  - Gap Junctions/metabolism
MH  - Mesenchymal Stem Cells/metabolism
MH  - Mice
MH  - Myocytes, Cardiac/*cytology/drug effects/metabolism
MH  - Rats
MH  - Stromal Cells/physiology
MH  - Up-Regulation
EDAT- 2010/08/31 06:00
MHDA- 2010/10/16 06:00
CRDT- 2010/08/31 06:00
PHST- 2010/03/12 00:00 [received]
PHST- 2010/07/27 00:00 [revised]
PHST- 2010/07/28 00:00 [accepted]
PHST- 2010/08/31 06:00 [entrez]
PHST- 2010/08/31 06:00 [pubmed]
PHST- 2010/10/16 06:00 [medline]
AID - S0940-9602(10)00107-X [pii]
AID - 10.1016/j.aanat.2010.07.001 [doi]
PST - ppublish
SO  - Ann Anat. 2010 Sep 20;192(5):314-21. doi: 10.1016/j.aanat.2010.07.001. Epub 2010 
      Aug 5.