PMID- 20801640
OWN - NLM
STAT- MEDLINE
DCOM- 20110509
LR  - 20101129
IS  - 1879-0852 (Electronic)
IS  - 0959-8049 (Linking)
VI  - 46
IP  - 18
DP  - 2010 Dec
TI  - Downregulation of miR-21 modulates Ras expression to promote apoptosis and 
      suppress invasion of Laryngeal squamous cell carcinoma.
PG  - 3409-16
LID - 10.1016/j.ejca.2010.07.047 [doi]
AB  - MiRNAs are small, noncoding RNA molecules that emerge as important regulators of 
      cancer-related processes. The miR-21 microRNA is overexpressed in a wide variety 
      of cancers and has been causally related to cellular proliferation and apoptosis. 
      In this study, we found that miR-21 is overexpressed in Laryngeal squamous cell 
      carcinoma (LSCC) and correlated with advanced stage. Inhibition of miR-21 by 
      antisense oligonucleotides (ASO) led to decreased protein level of Ras and 
      profound suppression of cell proliferation and invasion. Hep-2 cells exposed to 
      miR-21 ASO exhibited cell cycle arrest at G1 phase and increased apoptosis. 
      Furthermore, growth of LSCC xenograft tumours was significantly suppressed by 
      repeated injection of ASO-miR-21 lentivirus and the Ras protein expression in 
      LSCC xenograft tumours was also downregulate by ASO-miR-21. Taken together, our 
      data suggest that miR-21 may play an oncogenic role in the cellular processes of 
      LSCC and represent a novel target for effective therapies.
CI  - Copyright (c) 2010 Elsevier Ltd. All rights reserved.
FAU - Ren, Jingyuan
AU  - Ren J
AD  - Department of Otorhinolaryngology, Head and Neck Surgery, The Second Affiliated 
      Hospital, Harbin Medical University, Harbin 150081, China.
FAU - Zhu, Dan
AU  - Zhu D
FAU - Liu, Ming
AU  - Liu M
FAU - Sun, Yanan
AU  - Sun Y
FAU - Tian, Linli
AU  - Tian L
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - Eur J Cancer
JT  - European journal of cancer (Oxford, England : 1990)
JID - 9005373
RN  - 0 (MIRN21 microRNA, human)
RN  - 0 (MicroRNAs)
RN  - 0 (Neoplasm Proteins)
RN  - EC 3.6.5.2 (Proto-Oncogene Proteins p21(ras))
SB  - IM
MH  - Animals
MH  - Apoptosis/*physiology
MH  - Carcinoma, Squamous Cell/*metabolism/pathology
MH  - Down-Regulation
MH  - Female
MH  - Humans
MH  - Laryngeal Neoplasms/*metabolism/pathology
MH  - Male
MH  - Mice
MH  - Mice, Inbred BALB C
MH  - MicroRNAs/*metabolism
MH  - Middle Aged
MH  - Neoplasm Invasiveness/physiopathology
MH  - Neoplasm Proteins/*metabolism
MH  - Proto-Oncogene Proteins p21(ras)/*metabolism
MH  - Tumor Cells, Cultured
EDAT- 2010/08/31 06:00
MHDA- 2011/05/10 06:00
CRDT- 2010/08/31 06:00
PHST- 2010/06/22 00:00 [received]
PHST- 2010/07/27 00:00 [revised]
PHST- 2010/07/29 00:00 [accepted]
PHST- 2010/08/31 06:00 [entrez]
PHST- 2010/08/31 06:00 [pubmed]
PHST- 2011/05/10 06:00 [medline]
AID - S0959-8049(10)00757-4 [pii]
AID - 10.1016/j.ejca.2010.07.047 [doi]
PST - ppublish
SO  - Eur J Cancer. 2010 Dec;46(18):3409-16. doi: 10.1016/j.ejca.2010.07.047.