PMID- 20802296
OWN - NLM
STAT- MEDLINE
DCOM- 20110325
LR  - 20111117
IS  - 1473-5571 (Electronic)
IS  - 0269-9370 (Linking)
VI  - 24
IP  - 15
DP  - 2010 Sep 24
TI  - Distinct differentiation profiles of HIV-Gag and Nef-specific central memory CD8+ 
      T cells associated with HLA-B57/5801 and virus control.
PG  - 2323-9
LID - 10.1097/QAD.0b013e32833e5009 [doi]
AB  - OBJECTIVES: A superior capacity of controlling HIV has been attributed to CD8(+) 
      T cells directed against HIV-Gag compared to Nef, particularly in the context of 
      some protective human leukocyte antigen (HLA) alleles. To further elucidate this 
      protective effect, we compared the multifunctional and differentiation 
      characteristics of CD8(+) T cells specific for HIV-Gag and Nef in 
      HLA-B57/5801-positive and negative nonprogressors. METHODS: A head-to-head 
      comparison of CD8(+) T cells specific for HIV-Gag and Nef frequencies, cytokine 
      production and differentiation was conducted, in 11 HLA-B57/5801 and 11 
      HLA-B57/5801 HIV-infected individuals selected from a cohort of 53 nonprogressors 
      by using IFN-gamma-ELISpot assay and flow cytometry analysis of intracellular 
      cytokine production and differentiation profile. Correlations with HIV parameters 
      were studied. RESULTS: Frequencies of Gag-specific but not of Nef-specific CD8(+) 
      T cells correlated with peripheral blood mononuclear cell (PBMC)-associated 
      HIV-DNA. The HIV-Gag and Nef-specific CD8(+) T cells did not differ for IL-2 
      production in either HLA-B57/5801 or HLA-B57/5801 individuals. The 
      IFN-gamma-producing Gag-specific CD8(+) T cells in HLA-B57/5801 individuals 
      significantly differed from their Nef-specific counterparts by displaying higher 
      proportions of central memory CD45RACCR7 cells positive for CD27(+). This 
      differentiation pattern was not observed in HLA-B57/5801 individuals. Only these 
      HLA-B57/5801-positive Gag-specific CD27(+) central memory CD8(+) T cells, but not 
      their Nef-specific counterparts, negatively correlated with cell-associated 
      HIV-DNA. CONCLUSION: HLA-B57/5801 drives a preferential CD27(+) differentiation 
      of central memory CD8(+) T cells directed against HIV-Gag but not Nef that may 
      contribute to the ability of Gag-specific CD8(+) T cells to better control HIV in 
      HLA-B57/5801 nonprogressors.
FAU - Xie, Jing
AU  - Xie J
AD  - Laboratoire d'Immunite et Infections, INSERM, UMR-S 945, Hopital 
      Pitie-Salpetriere, UPMC Univ Paris, Paris, France.
FAU - Lu, Wei
AU  - Lu W
FAU - Samri, Assia
AU  - Samri A
FAU - Costagliola, Dominique
AU  - Costagliola D
FAU - Schnuriger, Aurelie
AU  - Schnuriger A
FAU - da Silva, Bosco C M
AU  - da Silva BC
FAU - Blanc, Catherine
AU  - Blanc C
FAU - Larsen, Martin
AU  - Larsen M
FAU - Theodorou, Ioannis
AU  - Theodorou I
FAU - Rouzioux, Christine
AU  - Rouzioux C
FAU - Autran, Brigitte
AU  - Autran B
CN  - ALT-ANRS-CO15 study group
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - England
TA  - AIDS
JT  - AIDS (London, England)
JID - 8710219
RN  - 0 (DNA, Viral)
RN  - 0 (HLA-B Antigens)
RN  - 0 (HLA-B57 antigen)
RN  - 0 (nef Gene Products, Human Immunodeficiency Virus)
SB  - IM
MH  - CD8-Positive T-Lymphocytes/*immunology
MH  - Cell Separation
MH  - DNA, Viral/genetics
MH  - Enzyme-Linked Immunosorbent Assay
MH  - Genes, gag/*genetics
MH  - HIV Infections/genetics/*immunology
MH  - HIV-1/*genetics
MH  - HLA-B Antigens/*immunology
MH  - Humans
MH  - nef Gene Products, Human Immunodeficiency Virus/genetics/*immunology
FIR - Debre, P
IR  - Debre P
FIR - Almeida, M
IR  - Almeida M
FIR - Pelle, O
IR  - Pelle O
FIR - Clauvel, J P
IR  - Clauvel JP
FIR - Sicard, D
IR  - Sicard D
EDAT- 2010/08/31 06:00
MHDA- 2011/03/26 06:00
CRDT- 2010/08/31 06:00
PHST- 2010/08/31 06:00 [entrez]
PHST- 2010/08/31 06:00 [pubmed]
PHST- 2011/03/26 06:00 [medline]
AID - 10.1097/QAD.0b013e32833e5009 [doi]
PST - ppublish
SO  - AIDS. 2010 Sep 24;24(15):2323-9. doi: 10.1097/QAD.0b013e32833e5009.