PMID- 20804613
OWN - NLM
STAT- MEDLINE
DCOM- 20110201
LR  - 20211020
IS  - 1423-0127 (Electronic)
IS  - 1021-7770 (Print)
IS  - 1021-7770 (Linking)
VI  - 17 Suppl 1
IP  - Suppl 1
DP  - 2010 Aug 24
TI  - Regulation of taurine transport at the blood-placental barrier by calcium ion, 
      PKC activator and oxidative stress conditions.
PG  - S37
LID - 10.1186/1423-0127-17-S1-S37 [doi]
AB  - BACKGROUND: In the present study, we investigated the changes of uptake and 
      efflux transport of taurine under various stress conditions using rat 
      conditionally immortalized syncytiotrophoblast cell line (TR-TBT cells), as in 
      vitro blood-placental barrier (BPB) model. METHODS: The transport of taurine in 
      TR-TBT cells were characterized by cellular uptake study using radiolabeled 
      taurine. The efflux of taurine was measured from the amount of radiolabeled 
      taurine remaining in the cells after the uptake of radiolabeled taurine for 60 
      min. RESULTS: Taurine uptake was significantly decreased by phosphorylation of 
      protein kinase C (PKC) activator in TR-TBT cells. Also, calcium ion (Ca2+) was 
      involved in taurine transport in TR-TBT cells. Taurine uptake was inhibited and 
      efflux was enhanced under calcium free conditions in the cells. In addition, 
      oxidative stress induced the change of taurine transport in TR-TBT cells, but the 
      changes were different depending on the types of oxidative stress inducing 
      agents. Tumor necrosis factor-alpha (TNF-alpha), lipopolysaccharide (LPS) and 
      diethyl maleate (DEM) significantly increased taurine uptake, but H2O2 and nitric 
      oxide (NO) donor decreased taurine uptake in the cells. Taurine efflux was 
      down-regulated by TNF-alpha in TR-TBT cells. CONCLUSION: Taurine transport in 
      TR-TBT cells were regulated diversely at extracellular Ca2+ level, PKC activator 
      and oxidative stress conditions. It suggested that variable stresses affected the 
      taurine supplies from maternal blood to fetus and taurine level of fetus.
FAU - Lee, Na-Young
AU  - Lee NY
AD  - College of Pharmacy and Research Institute of Pharmaceutical Science, Sookmyung 
      Women's University, Seoul, 140-742, Republic of Korea. nylee0@sookmyung.ac.kr
FAU - Kang, Young-Sook
AU  - Kang YS
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100824
PL  - England
TA  - J Biomed Sci
JT  - Journal of biomedical science
JID - 9421567
RN  - 0 (Calcium Channel Blockers)
RN  - 1EQV5MLY3D (Taurine)
RN  - EC 2.7.11.13 (Protein Kinase C)
RN  - SY7Q814VUP (Calcium)
SB  - IM
MH  - Animals
MH  - Biological Transport/physiology
MH  - Calcium/*metabolism
MH  - Calcium Channel Blockers/metabolism
MH  - Cell Line
MH  - Enzyme Activation
MH  - Female
MH  - *Oxidative Stress
MH  - Placenta/*metabolism
MH  - Pregnancy
MH  - Protein Kinase C/*metabolism
MH  - Rats
MH  - Taurine/*metabolism
PMC - PMC2994386
EDAT- 2010/09/11 06:00
MHDA- 2011/02/02 06:00
PMCR- 2010/08/24
CRDT- 2010/09/01 06:00
PHST- 2010/09/01 06:00 [entrez]
PHST- 2010/09/11 06:00 [pubmed]
PHST- 2011/02/02 06:00 [medline]
PHST- 2010/08/24 00:00 [pmc-release]
AID - 1423-0127-17-S1-S37 [pii]
AID - 10.1186/1423-0127-17-S1-S37 [doi]
PST - epublish
SO  - J Biomed Sci. 2010 Aug 24;17 Suppl 1(Suppl 1):S37. doi: 
      10.1186/1423-0127-17-S1-S37.