PMID- 20804756
OWN - NLM
STAT- MEDLINE
DCOM- 20101029
LR  - 20220317
IS  - 1873-3468 (Electronic)
IS  - 0014-5793 (Linking)
VI  - 584
IP  - 19
DP  - 2010 Oct 8
TI  - The density of extracellular matrix proteins regulates inflammation and insulin 
      signaling in adipocytes.
PG  - 4145-50
LID - 10.1016/j.febslet.2010.08.033 [doi]
AB  - Cells can not only sense the type of extracellular matrix (ECM) protein that is 
      present in the microenvironment, but they can also sense its density. Here, we 
      investigated the effects of ECM protein density on adipokine secretion and 
      insulin signaling in adipocytes. To this end, 3T3-L1 adipocytes were cultured on 
      the surface of polyacrylamide gels that were coated with gradient densities of a 
      collagen type I and fibronectin mixture. We found that high density ECM causes a 
      decrease in insulin signaling and adiponectin secretion, whereas the secretion of 
      monocyte chemoattractant protein-1 (MCP-1) was increased via the activation of 
      nuclear factor-kappaB (NF-kappaB). These results indicate that the density of the ECM 
      directly regulates the inflammatory response and insulin sensitivity of 
      adipocytes.
CI  - Copyright (c) 2010 Federation of European Biochemical Societies. Published by 
      Elsevier B.V. All rights reserved.
FAU - Li, Qinkai
AU  - Li Q
AD  - Clinical Research Center for Diabetes, Tokushima University Hospital, Tokushima, 
      Japan. lqk@clin.med.tokushima-u.ac.jp
FAU - Hata, Akiko
AU  - Hata A
FAU - Kosugi, Chisato
AU  - Kosugi C
FAU - Kataoka, Nananko
AU  - Kataoka N
FAU - Funaki, Makoto
AU  - Funaki M
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100915
PL  - England
TA  - FEBS Lett
JT  - FEBS letters
JID - 0155157
RN  - 0 (Adiponectin)
RN  - 0 (Adipoq protein, mouse)
RN  - 0 (Ccl2 protein, mouse)
RN  - 0 (Chemokine CCL2)
RN  - 0 (Extracellular Matrix Proteins)
RN  - 0 (Insulin)
RN  - 0 (NF-kappa B)
SB  - IM
MH  - 3T3-L1 Cells
MH  - Adipocytes/*metabolism
MH  - Adiponectin/metabolism
MH  - Animals
MH  - Chemokine CCL2/metabolism
MH  - Extracellular Matrix Proteins/*metabolism
MH  - Inflammation/*metabolism
MH  - Insulin/*metabolism
MH  - Insulin Resistance
MH  - Mice
MH  - NF-kappa B/metabolism
MH  - Signal Transduction
EDAT- 2010/09/02 06:00
MHDA- 2010/10/30 06:00
CRDT- 2010/09/01 06:00
PHST- 2010/08/03 00:00 [received]
PHST- 2010/08/23 00:00 [revised]
PHST- 2010/08/23 00:00 [accepted]
PHST- 2010/09/01 06:00 [entrez]
PHST- 2010/09/02 06:00 [pubmed]
PHST- 2010/10/30 06:00 [medline]
AID - S0014-5793(10)00695-2 [pii]
AID - 10.1016/j.febslet.2010.08.033 [doi]
PST - ppublish
SO  - FEBS Lett. 2010 Oct 8;584(19):4145-50. doi: 10.1016/j.febslet.2010.08.033. Epub 
      2010 Sep 15.