PMID- 20804797
OWN - NLM
STAT- MEDLINE
DCOM- 20110210
LR  - 20211203
IS  - 1873-5169 (Electronic)
IS  - 0196-9781 (Print)
IS  - 0196-9781 (Linking)
VI  - 31
IP  - 12
DP  - 2010 Dec
TI  - Regulation of gastric hormones by systemic rapamycin.
PG  - 2185-92
LID - 10.1016/j.peptides.2010.08.018 [doi]
AB  - The mammalian target of rapamycin (mTOR), an evolutionarily conserved 
      serine-threonine kinase, is an intracellular fuel sensor critical for cellular 
      energy homeostasis. Gastrointestinal endocrine cells play a vital role in the 
      regulation of energy balance by secreting hormones that inform the brain about 
      energy supply. Here we showed the localization of mTOR signaling molecules in 
      more than 90% of gastric ghrelin cells and 36+/-3% of gastrin cells, while no 
      somatostatin-positive cell showed phospho-S6K1 immunoreactivity. Inhibition of 
      mTOR significantly stimulated expression of gastric ghrelin mRNA and protein, and 
      the concentration of plasma ghrelin (2.06+/-0.34 ng/ml vs. 12.53+/-3.9 ng/ml, 
      p<0.05), inhibited gastrin synthesis and secretion (75.01+/-6.71 pg/ml vs. 
      54.04+/-3.65 pg/ml, p<0.05), but had no effect on somatostatin production 
      (165.2+/-25.07 pg/ml vs. 178.9+/-29.14 pg/ml, p=0.73). Gastric mTOR is a gastric 
      sensor whose activity is linked to the differential regulation of gastric hormone 
      production and release.
CI  - Copyright (c) 2010 Elsevier Inc. All rights reserved.
FAU - Xu, Geyang
AU  - Xu G
AD  - Department of Physiology and Pathophysiology, Peking University Health Science 
      Center, Beijing 100191, China.
FAU - Li, Yin
AU  - Li Y
FAU - An, Wenjiao
AU  - An W
FAU - Zhao, Jing
AU  - Zhao J
FAU - Xiang, Xinxin
AU  - Xiang X
FAU - Ding, Li
AU  - Ding L
FAU - Li, Ziru
AU  - Li Z
FAU - Guan, Youfei
AU  - Guan Y
FAU - Wang, Xian
AU  - Wang X
FAU - Tang, Chaoshu
AU  - Tang C
FAU - Zhu, Yi
AU  - Zhu Y
FAU - Wang, Nanping
AU  - Wang N
FAU - Li, Xiaoying
AU  - Li X
FAU - Mulholland, Michael
AU  - Mulholland M
FAU - Zhang, Weizhen
AU  - Zhang W
LA  - eng
GR  - R01 DK043225/DK/NIDDK NIH HHS/United States
GR  - R37 DK043225/DK/NIDDK NIH HHS/United States
GR  - R01DK043225/DK/NIDDK NIH HHS/United States
GR  - R01 DK054032/DK/NIDDK NIH HHS/United States
GR  - R01 DK043225-13/DK/NIDDK NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100908
PL  - United States
TA  - Peptides
JT  - Peptides
JID - 8008690
RN  - 0 (Gastrins)
RN  - 0 (Ghrelin)
RN  - 51110-01-1 (Somatostatin)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
RN  - W36ZG6FT64 (Sirolimus)
SB  - IM
MH  - Animals
MH  - Blotting, Western
MH  - Enteroendocrine Cells/drug effects/metabolism
MH  - Gastrins/*blood/genetics/metabolism
MH  - Ghrelin/*blood/genetics/metabolism
MH  - Humans
MH  - Immunohistochemistry
MH  - Male
MH  - Mice
MH  - Mice, Inbred C57BL
MH  - Polymerase Chain Reaction
MH  - Rats
MH  - Sirolimus/*pharmacology
MH  - Somatostatin/*blood/genetics/metabolism
MH  - TOR Serine-Threonine Kinases/genetics/metabolism
PMC - PMC2995266
MID - NIHMS232852
EDAT- 2010/09/02 06:00
MHDA- 2011/02/11 06:00
PMCR- 2011/12/01
CRDT- 2010/09/01 06:00
PHST- 2010/06/18 00:00 [received]
PHST- 2010/08/20 00:00 [revised]
PHST- 2010/08/20 00:00 [accepted]
PHST- 2010/09/01 06:00 [entrez]
PHST- 2010/09/02 06:00 [pubmed]
PHST- 2011/02/11 06:00 [medline]
PHST- 2011/12/01 00:00 [pmc-release]
AID - S0196-9781(10)00358-X [pii]
AID - 10.1016/j.peptides.2010.08.018 [doi]
PST - ppublish
SO  - Peptides. 2010 Dec;31(12):2185-92. doi: 10.1016/j.peptides.2010.08.018. Epub 2010 
      Sep 8.