PMID- 20804801
OWN - NLM
STAT- MEDLINE
DCOM- 20110505
LR  - 20121115
IS  - 1879-1166 (Electronic)
IS  - 0198-8859 (Linking)
VI  - 71
IP  - 11
DP  - 2010 Nov
TI  - Immunologic properties of human dermal fibroblasts.
PG  - 1089-98
LID - 10.1016/j.humimm.2010.08.003 [doi]
AB  - Human dermal fibroblasts are known not to express human leukocyte antigen 
      (HLA)-DR message or protein in the absence of interferon (IFN)-gamma. To use 
      allogeneic dermal fibroblasts for cell therapy, as a revalidation, the cells at 
      passage 12 were analyzed for HLA-DR mRNA and surface protein. Although no 
      significant HLA-DR surface protein was found, HLA-DR mRNA expression was 
      observed, without interferon-gamma. At an early passage, although HLA-DR surface 
      protein was not found, prominent expression of HLA-DR mRNA was observed without 
      IFN-gamma stimulation, which was not typical of dermal fibroblasts studied so far. 
      Intracytoplasmic HLA-DR protein was also not detected, which suggests that the 
      mRNA was not translated. There was no marked stimulation of T-cell proliferation 
      by the fibroblasts in the absence or presence of IFN-gamma. Interestingly, 
      indoleamine dioxygenase, a molecule involved in immunosuppression, was also 
      expressed in dermal fibroblasts in the absence of IFN-gamma.
CI  - Copyright (c) 2010 American Society for Histocompatibility and Immunogenetics. 
      Published by Elsevier Inc. All rights reserved.
FAU - Deshpande, Manisha
AU  - Deshpande M
AD  - Reliance Life Sciences Pvt Ltd., Dhirubhai Ambani Life Sciences Centre, 
      Maharashtra, India. manisha_deshpande@relbio.com
FAU - Tipnis, Shabari
AU  - Tipnis S
FAU - Shetty, Prathibha
AU  - Shetty P
FAU - Ghosh, Deepa
AU  - Ghosh D
FAU - Viswanathan, Chandra
AU  - Viswanathan C
FAU - Majumdar, Anish Sen
AU  - Majumdar AS
LA  - eng
PT  - Journal Article
DEP - 20100917
PL  - United States
TA  - Hum Immunol
JT  - Human immunology
JID - 8010936
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Indoleamine-Pyrrole 2,3,-Dioxygenase)
RN  - 0 (RNA, Messenger)
RN  - 82115-62-6 (Interferon-gamma)
SB  - IM
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - Dermis/*pathology
MH  - Fibroblasts/immunology/*metabolism/pathology
MH  - Foreskin/pathology
MH  - HLA-DR Antigens/genetics/immunology/*metabolism
MH  - Humans
MH  - Immune Tolerance
MH  - Indoleamine-Pyrrole 2,3,-Dioxygenase/metabolism
MH  - Infant, Newborn
MH  - Interferon-gamma/immunology/metabolism
MH  - Lymphocyte Activation
MH  - Male
MH  - RNA, Messenger/*analysis
MH  - T-Lymphocytes/immunology/*metabolism/pathology
EDAT- 2010/09/02 06:00
MHDA- 2011/05/06 06:00
CRDT- 2010/09/01 06:00
PHST- 2010/01/06 00:00 [received]
PHST- 2010/07/21 00:00 [revised]
PHST- 2010/08/16 00:00 [accepted]
PHST- 2010/09/01 06:00 [entrez]
PHST- 2010/09/02 06:00 [pubmed]
PHST- 2011/05/06 06:00 [medline]
AID - S0198-8859(10)00471-4 [pii]
AID - 10.1016/j.humimm.2010.08.003 [doi]
PST - ppublish
SO  - Hum Immunol. 2010 Nov;71(11):1089-98. doi: 10.1016/j.humimm.2010.08.003. Epub 
      2010 Sep 17.