PMID- 20805129
OWN - NLM
STAT- MEDLINE
DCOM- 20110314
LR  - 20101124
IS  - 1468-2079 (Electronic)
IS  - 0007-1161 (Linking)
VI  - 94
IP  - 12
DP  - 2010 Dec
TI  - Characterisation of the phenotype and function of monocyte-derived dendritic 
      cells in allergic conjunctiva.
PG  - 1662-7
LID - 10.1136/bjo.2009.177774 [doi]
AB  - BACKGROUND: Dendritic cells (DCs) are the most potent antigen-presenting cells 
      involved in initiating the immune response, presenting antigens to T cells and 
      leading to T cell proliferation. In an immature state, DCs lack accessory signals 
      required for T cell stimulation but are highly specialised to capture antigens. 
      Full DC maturation changes the cell surface phenotype and facilitates stimulation 
      of T cell proliferative responses. To examine the degree of DC maturity 
      associated with vernal keratoconjunctivitis (VKC), the authors examined the 
      phenotype and antigen-presentation capability of blood derived DCs from VKC 
      patients and from normal controls. METHODS: Flow cytometry was used to identify 
      the cell surface expression of markers of DC maturity (CD83, CD86, major 
      histocompatibility complex class II) and mixed leucocyte reactions to assess DC 
      induction of T cell proliferation. RESULTS: DCs derived from VKC patients were of 
      a more mature phenotype than those from normal controls. However, these VKC DCs 
      had reduced capability for induction of T cell proliferation compared with DCs 
      from controls. CONCLUSION: The increased maturity of DCs in VKC patients 
      correlates with the heightened immune responsiveness associated with this 
      disorder. A number of mechanisms may underlie the impaired ability of DCs in 
      atopy to stimulate T cell proliferation. This impairment of DC induction of T 
      cell activation is likely to be one factor which contributes to the modified 
      inflammatory response seen in VKC patients and the recognised susceptibility of 
      these patients to viral infection.
FAU - Manzouri, Bita
AU  - Manzouri B
AD  - UCL Institute of Ophthalmology, London, UK. bita.manzouri@moorfields.nhs.uk
FAU - Ohbayashi, Masaharu
AU  - Ohbayashi M
FAU - Leonardi, Andrea
AU  - Leonardi A
FAU - Fattah, Dilniya
AU  - Fattah D
FAU - Larkin, Daniel F P
AU  - Larkin DF
FAU - Ono, Santa J
AU  - Ono SJ
LA  - eng
GR  - G78/7987/Medical Research Council/United Kingdom
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100830
PL  - England
TA  - Br J Ophthalmol
JT  - The British journal of ophthalmology
JID - 0421041
RN  - 0 (Antibodies, Monoclonal)
SB  - IM
MH  - Antibodies, Monoclonal/immunology
MH  - Conjunctivitis, Allergic/genetics/*immunology
MH  - Dendritic Cells/*cytology/*immunology
MH  - Donor Selection
MH  - Flow Cytometry
MH  - Humans
MH  - Lymphocyte Activation
MH  - Phenotype
MH  - T-Lymphocytes/cytology/*immunology
EDAT- 2010/09/02 06:00
MHDA- 2011/03/15 06:00
CRDT- 2010/09/01 06:00
PHST- 2010/09/01 06:00 [entrez]
PHST- 2010/09/02 06:00 [pubmed]
PHST- 2011/03/15 06:00 [medline]
AID - bjo.2009.177774 [pii]
AID - 10.1136/bjo.2009.177774 [doi]
PST - ppublish
SO  - Br J Ophthalmol. 2010 Dec;94(12):1662-7. doi: 10.1136/bjo.2009.177774. Epub 2010 
      Aug 30.