PMID- 20805842
OWN - NLM
STAT- MEDLINE
DCOM- 20110812
LR  - 20180822
IS  - 1440-1711 (Electronic)
IS  - 0818-9641 (Linking)
VI  - 89
IP  - 3
DP  - 2011 Mar
TI  - The lymphoid chemokine CCL21 triggers LFA-1 adhesive properties on human 
      dendritic cells.
PG  - 458-65
LID - 10.1038/icb.2010.103 [doi]
AB  - Dendritic cells (DCs) are the most potent APCs, involved in the induction of 
      immunity and tolerance. Recently we showed that during differentiation of human 
      DCs from monocyte precursors, Lymphocyte function-associated antigen-1 
      (LFA-1)-binding capacity is lost, although integrin expression levels were 
      maintained constant, suggesting a different regulation mechanism of this integrin 
      on different cell types. However, the exact role of LFA-1 in DC adhesion and 
      migration remains obscure. Chemokines are potent regulators of integrin function, 
      influencing migratory and adhesive properties of leukocytes. Here, we show that 
      upon vaccination of cancer patients with human DCs, cells that have migrated in 
      vivo into the lymph nodes upregulated the active form of LFA-1. We further show 
      that exposure of human DCs to the lymphoid chemokine CCL21 specifically restores 
      the high-affinity form of LFA-1 and induces binding to its ligand ICAM-1 under 
      low shear stress. Our data indicate that on DCs LFA-1 may function as an 
      inducible anchor during lymphatic transmigration or within the lymph nodes. A 
      thorough understanding of the adhesive events during the DC life cycle will help 
      to improve the outcome of DC-based antitumor clinical trials.
FAU - Eich, Christina
AU  - Eich C
AD  - Department of Tumor Immunology, Nijmegen Centre for Molecular Life Sciences, 
      Radboud University Nijmegen Medical Centre, Nijmegen, The Netherlands.
FAU - de Vries, I Jolanda M
AU  - de Vries IJ
FAU - Linssen, Peter C M
AU  - Linssen PC
FAU - de Boer, Annemiek
AU  - de Boer A
FAU - Boezeman, Jan B
AU  - Boezeman JB
FAU - Figdor, Carl G
AU  - Figdor CG
FAU - Cambi, Alessandra
AU  - Cambi A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100831
PL  - United States
TA  - Immunol Cell Biol
JT  - Immunology and cell biology
JID - 8706300
RN  - 0 (Chemokine CCL21)
RN  - 0 (Lymphocyte Function-Associated Antigen-1)
RN  - 126547-89-5 (Intercellular Adhesion Molecule-1)
SB  - IM
MH  - Cell Differentiation/immunology
MH  - Cell Movement/immunology
MH  - Chemokine CCL21/*immunology
MH  - Dendritic Cells/*immunology/*metabolism
MH  - Humans
MH  - Intercellular Adhesion Molecule-1/metabolism
MH  - Lymph Nodes/immunology/metabolism
MH  - Lymphocyte Function-Associated Antigen-1/genetics/*metabolism
MH  - Protein Binding/immunology
EDAT- 2010/09/02 06:00
MHDA- 2011/08/13 06:00
CRDT- 2010/09/01 06:00
PHST- 2010/09/01 06:00 [entrez]
PHST- 2010/09/02 06:00 [pubmed]
PHST- 2011/08/13 06:00 [medline]
AID - icb2010103 [pii]
AID - 10.1038/icb.2010.103 [doi]
PST - ppublish
SO  - Immunol Cell Biol. 2011 Mar;89(3):458-65. doi: 10.1038/icb.2010.103. Epub 2010 
      Aug 31.