PMID- 20806254
OWN - NLM
STAT- MEDLINE
DCOM- 20110328
LR  - 20101217
IS  - 1521-4028 (Electronic)
IS  - 0233-111X (Linking)
VI  - 50
IP  - 6
DP  - 2010 Dec
TI  - Optimization of laccase production by Pleurotus ostreatus IMI 395545 using the 
      Taguchi DOE methodology.
PG  - 548-56
LID - 10.1002/jobm.201000095 [doi]
AB  - Production of laccase using a submerged culture of Pleurotus orstreatus IMI 
      395545 was optimized by the Taguchi orthogonal array (OA) design of experiments 
      (DOE) methodology. This approach facilitates the study of the interactions of a 
      large number of variables spanned by factors and their settings, with a small 
      number of experiments, leading to considerable savings in time and cost for 
      process optimization. This methodology optimizes the number of impact factors and 
      enables to calculate their interaction in the production of industrial enzymes. 
      Eight factors, viz. glucose, yeast extract, malt extract, inoculum, mineral 
      solution, inducer (1 mM CuSO(4)) and amino acid (l-asparagine) at three levels and 
      pH at two levels, with an OA layout of L18 (2(1) x 3(7)) were selected for the 
      proposed experimental design. The laccase yield obtained from the 18 sets of 
      fermentation experiments performed with the selected factors and levels was 
      further processed with Qualitek-4 software. The optimized conditions shared an 
      enhanced laccase expression of 86.8% (from 485.0 to 906.3 U). The combination of 
      factors was further validated for laccase production and reactive blue 221 
      decolorization. The results revealed an enhanced laccase yield of 32.6% and dye 
      decolorization up to 84.6%. This methodology allows the complete evaluation of 
      main and interaction factors.
CI  - (c) 2010 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim
FAU - Periasamy, Rathinasamy
AU  - Periasamy R
AD  - Department of Biochemistry, Periyar University, Salem, Tamil Nadu, India.
FAU - Palvannan, Thayumanavan
AU  - Palvannan T
LA  - eng
PT  - Journal Article
PL  - Germany
TA  - J Basic Microbiol
JT  - Journal of basic microbiology
JID - 8503885
RN  - 0 (Culture Media)
RN  - EC 1.10.3.2 (Laccase)
SB  - IM
MH  - Bioreactors/microbiology
MH  - Biotechnology/methods
MH  - Culture Media/chemistry
MH  - Hydrogen-Ion Concentration
MH  - Laccase/*biosynthesis
MH  - Pleurotus/*enzymology/growth & development/*metabolism
EDAT- 2010/09/02 06:00
MHDA- 2011/03/29 06:00
CRDT- 2010/09/01 06:00
PHST- 2010/09/01 06:00 [entrez]
PHST- 2010/09/02 06:00 [pubmed]
PHST- 2011/03/29 06:00 [medline]
AID - 10.1002/jobm.201000095 [doi]
PST - ppublish
SO  - J Basic Microbiol. 2010 Dec;50(6):548-56. doi: 10.1002/jobm.201000095.