PMID- 20806935
OWN - NLM
STAT- MEDLINE
DCOM- 20110114
LR  - 20220318
IS  - 1520-5118 (Electronic)
IS  - 0021-8561 (Linking)
VI  - 58
IP  - 18
DP  - 2010 Sep 22
TI  - Fast and simple droplet sampling of sap from plant tissues and capillary 
      microextraction of soluble saccharides for picogram-scale quantitative 
      determination with GC-MS.
PG  - 9931-5
LID - 10.1021/jf102053h [doi]
AB  - Soluble saccharides are very important metabolites of the life cycle and 
      synthesis of structural polysaccharide components (cellulose, hemicellulose, 
      pectin, etc.) of cell walls. A new method for droplet sampling of saps from 
      tissues of organisms and manipulation routines in capillaries for extraction, 
      derivation, and partitioning were developed for picogram-scale quantitative 
      determination with gas chromatography-mass spectrometry (GC-MS). Five to ten 
      microliters of sap was sampled with a glass capillary containing ribitol 
      (internal standard). Subsequently, the analytes were acetylated with acetic 
      anhydride and catalyzed by 1-methylimidazole. Finally, the soluble saccharides 
      were qualitatively detected with GC-MS SIM (selective ion monitoring) mode. The 
      linear ranges of the method were up to 1x10(-6) mol/L and the theoretically 
      lowest limits of detection (LOD, s/n>/=3) were up to 1x10(-9) mol/L. The method is 
      suitable and applicable to analysis of soluble monosaccharides in fresh tissues 
      and other aqueous samples in wide fields of agriculture, food science, biological 
      sciences, and even medical studies.
FAU - Tan, Yupeng
AU  - Tan Y
AD  - College of Biological Sciences and Biotechnology, Beijing Forestry University, 
      No. 35, Qinghua Donglu, Beijing 100083, People's Republic of China.
FAU - Li, Ke
AU  - Li K
FAU - Hu, Lei
AU  - Hu L
FAU - Chen, Shu
AU  - Chen S
FAU - Gai, Ying
AU  - Gai Y
FAU - Jiang, Xiangning
AU  - Jiang X
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - J Agric Food Chem
JT  - Journal of agricultural and food chemistry
JID - 0374755
RN  - 0 (Monosaccharides)
RN  - 0 (Oligosaccharides)
RN  - 0 (Plant Extracts)
RN  - 0 (Plant Exudates)
RN  - 0 (Sugar Alcohols)
SB  - IM
MH  - Analytic Sample Preparation Methods
MH  - Gas Chromatography-Mass Spectrometry
MH  - Microchemistry/*methods
MH  - Monosaccharides/*analysis/chemistry/isolation & purification
MH  - Oligosaccharides/*analysis/chemistry/isolation & purification
MH  - Plant Extracts/chemistry/isolation & purification
MH  - Plant Exudates/*chemistry
MH  - Solubility
MH  - Sugar Alcohols/analysis/chemistry/isolation & purification
EDAT- 2010/09/03 06:00
MHDA- 2011/01/15 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2011/01/15 06:00 [medline]
AID - 10.1021/jf102053h [doi]
PST - ppublish
SO  - J Agric Food Chem. 2010 Sep 22;58(18):9931-5. doi: 10.1021/jf102053h.