PMID- 20807557
OWN - NLM
STAT- MEDLINE
DCOM- 20110124
LR  - 20191210
IS  - 1872-8359 (Electronic)
IS  - 0167-7012 (Linking)
VI  - 83
IP  - 2
DP  - 2010 Nov
TI  - Fluorescence in situ hybridization rapidly detects three different pathogenic 
      bacteria in urinary tract infection samples.
PG  - 175-8
LID - 10.1016/j.mimet.2010.08.015 [doi]
AB  - The detection of pathogenic bacteria in urine is an important criterion for 
      diagnosing urinary tract infections (UTIs). By using fluorescence in situ 
      hybridization (FISH) with rRNA-targeted, fluorescently labeled oligonucleotide 
      probes, bacterial pathogens present in urine samples were identified within 3-4 
      h. In this study, three probes that are specific for Escherichia coli, 
      Enterococcus faecalis and Staphylococcus aureus were designed based on the 
      conserved 16S RNA sequences, whereas probe Eub338 broadly recognizes all 
      bacteria. We collected a total of 1000 urine samples, and 325 of these samples 
      tested positive for a UTI via traditional culturing techniques; additionally, all 
      325 of these samples tested positive with the Eub338 probe in FISH analysis. FISH 
      analyses with species-specific probes were performed in parallel to the test the 
      ability to differentiate among several pathogenic bacteria. The samples for these 
      experiments included 76 E. coli infected samples, 32 E. faecalis infected samples 
      and 9 S. aureus infected samples. Compared to conventional methods of bacterial 
      identification, the FISH method produced positive results for >90% of the samples 
      tested. FISH has the potential to become an extremely useful diagnostic tool for 
      UTIs because it has a quick turnaround time and high accuracy.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Wu, Qing
AU  - Wu Q
AD  - Department of Clinical Laboratory, Renmin Hospital of Wuhan University, Jiefang 
      Road 238, Wuhan, 430060, Hubei Province, China.
FAU - Li, Yan
AU  - Li Y
FAU - Wang, Ming
AU  - Wang M
FAU - Pan, Xiao P
AU  - Pan XP
FAU - Tang, Yong F
AU  - Tang YF
LA  - eng
PT  - Evaluation Study
PT  - Journal Article
DEP - 20100831
PL  - Netherlands
TA  - J Microbiol Methods
JT  - Journal of microbiological methods
JID - 8306883
RN  - 0 (Oligonucleotide Probes)
RN  - 0 (RNA, Ribosomal)
SB  - IM
MH  - Bacteriological Techniques/*methods
MH  - Enterococcus faecalis/genetics/isolation & purification
MH  - Escherichia coli/genetics/isolation & purification
MH  - Escherichia coli Infections/*diagnosis/microbiology
MH  - Gram-Positive Bacterial Infections/*diagnosis/microbiology
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Oligonucleotide Probes/genetics
MH  - RNA, Ribosomal/genetics
MH  - Sensitivity and Specificity
MH  - Staphylococcus aureus/genetics/isolation & purification
MH  - Urinary Tract Infections/*microbiology
EDAT- 2010/09/03 06:00
MHDA- 2011/01/25 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/04/08 00:00 [received]
PHST- 2010/08/17 00:00 [revised]
PHST- 2010/08/17 00:00 [accepted]
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2011/01/25 06:00 [medline]
AID - S0167-7012(10)00281-2 [pii]
AID - 10.1016/j.mimet.2010.08.015 [doi]
PST - ppublish
SO  - J Microbiol Methods. 2010 Nov;83(2):175-8. doi: 10.1016/j.mimet.2010.08.015. Epub 
      2010 Aug 31.