PMID- 20807763
OWN - NLM
STAT- MEDLINE
DCOM- 20101130
LR  - 20211020
IS  - 1083-351X (Electronic)
IS  - 0021-9258 (Print)
IS  - 0021-9258 (Linking)
VI  - 285
IP  - 45
DP  - 2010 Nov 5
TI  - The carboxyl-terminal end of Cox1 is required for feedback assembly regulation of 
      Cox1 synthesis in Saccharomyces cerevisiae mitochondria.
PG  - 34382-9
LID - 10.1074/jbc.M110.161976 [doi]
AB  - Synthesis of the largest cytochrome c oxidase (CcO) subunit, Cox1, on yeast 
      mitochondrial ribosomes is coupled to assembly of CcO. The translational 
      activator Mss51 is sequestered in early assembly intermediate complexes by an 
      interaction with Cox14 that depends on the presence of newly synthesized Cox1. If 
      CcO assembly is prevented, the level of Mss51 available for translational 
      activation is reduced. We deleted the C-terminal 11 or 15 residues of Cox1 by 
      site-directed mutagenesis of mtDNA. Although these deletions did not prevent 
      respiratory growth of yeast, they eliminated the assembly-feedback control of 
      Cox1 synthesis. Furthermore, these deletions reduced the strength of the 
      Mss51-Cox14 interaction as detected by co-immunoprecipitation, confirming the 
      importance of the Cox1 C-terminal residues for Mss51 sequestration. We surveyed a 
      panel of mutations that block CcO assembly for the strength of their effect on 
      Cox1 synthesis, both by pulse labeling and expression of the ARG8(m) reporter 
      fused to COX1. Deletion of the nuclear gene encoding Cox6, one of the first 
      subunits to be added to assembling CcO, caused the most severe reduction in Cox1 
      synthesis. Deletion of the C-terminal 15 amino acids of Cox1 increased Cox1 
      synthesis in the presence of each of these mutations, except pet54. Our data 
      suggest a novel activity of Pet54 required for normal synthesis of Cox1 that is 
      independent of the Cox1 C-terminal end.
FAU - Shingu-Vazquez, Miguel
AU  - Shingu-Vazquez M
AD  - Departamento de Genetica Molecular, Instituto de Fisiologia Celular, Universidad 
      Nacional Autonoma de Mexico, Mexico DF 04510, Mexico.
FAU - Camacho-Villasana, Yolanda
AU  - Camacho-Villasana Y
FAU - Sandoval-Romero, Luisa
AU  - Sandoval-Romero L
FAU - Butler, Christine A
AU  - Butler CA
FAU - Fox, Thomas D
AU  - Fox TD
FAU - Perez-Martinez, Xochitl
AU  - Perez-Martinez X
LA  - eng
GR  - R01 GM029362/GM/NIGMS NIH HHS/United States
GR  - R01 GM029362-29/GM/NIGMS NIH HHS/United States
GR  - GM029362/GM/NIGMS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PT  - Research Support, Non-U.S. Gov't
DEP - 20100831
PL  - United States
TA  - J Biol Chem
JT  - The Journal of biological chemistry
JID - 2985121R
RN  - 0 (COX14 protein, S cerevisiae)
RN  - 0 (Membrane Proteins)
RN  - 0 (Mitochondrial Proteins)
RN  - 0 (Mss51 protein, S cerevisiae)
RN  - 0 (Pet54 protein, S cerevisiae)
RN  - 0 (RNA-Binding Proteins)
RN  - 0 (Saccharomyces cerevisiae Proteins)
RN  - 0 (Transcription Factors)
RN  - EC 1.9.3.1 (Cox1 protein, S cerevisiae)
RN  - EC 1.9.3.1 (Electron Transport Complex IV)
SB  - IM
MH  - Amino Acid Sequence
MH  - Electron Transport Complex IV/*biosynthesis/genetics
MH  - Membrane Proteins/genetics/metabolism
MH  - Mitochondria/*enzymology/genetics
MH  - Mitochondrial Proteins/genetics/metabolism
MH  - RNA-Binding Proteins/genetics/metabolism
MH  - Saccharomyces cerevisiae/*enzymology/genetics/growth & development
MH  - Saccharomyces cerevisiae Proteins/*biosynthesis/genetics/metabolism
MH  - Sequence Deletion
MH  - Transcription Factors/genetics/metabolism
MH  - Transcription, Genetic/physiology
PMC - PMC2966052
EDAT- 2010/09/03 06:00
MHDA- 2010/12/14 06:00
PMCR- 2011/11/05
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/14 06:00 [medline]
PHST- 2011/11/05 00:00 [pmc-release]
AID - S0021-9258(20)46978-9 [pii]
AID - M110.161976 [pii]
AID - 10.1074/jbc.M110.161976 [doi]
PST - ppublish
SO  - J Biol Chem. 2010 Nov 5;285(45):34382-9. doi: 10.1074/jbc.M110.161976. Epub 2010 
      Aug 31.