PMID- 20809308
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20100902
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 659
DP  - 2010
TI  - The Comet-FISH assay for the analysis of DNA damage and repair.
PG  - 129-45
LID - 10.1007/978-1-60761-789-1_9 [doi]
AB  - In this chapter, I describe the alkaline single-cell gel electrophoresis (Comet 
      assay) combined with fluorescence in situ hybridization (FISH) technology, used 
      in our laboratory, to study the incidence and repair of lesions induced in human 
      cells by ultraviolet light. The Comet-FISH method permits the simultaneous and 
      comparative analysis of DNA damage and its repair throughout the genome and in 
      defined chromosomal regions. This very sensitive approach can be applied to any 
      lesion, such as those induced by chemical carcinogens and products of cellular 
      metabolism that can be converted to DNA single- or double-strand breaks. The 
      unique advantages and limitations of the method for particular applications are 
      discussed.
FAU - Spivak, Graciela
AU  - Spivak G
AD  - Department of Biology, Stanford University, Stanford, CA, USA. 
      gspivak@stanford.edu
LA  - eng
GR  - CA91456/CA/NCI NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Enzymes)
SB  - IM
MH  - Comet Assay/*methods
MH  - *DNA Damage
MH  - *DNA Repair
MH  - Enzymes/metabolism
MH  - Fibroblasts/cytology/metabolism/radiation effects
MH  - Humans
MH  - Image Processing, Computer-Assisted
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Microscopy, Fluorescence
EDAT- 2010/09/03 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - 10.1007/978-1-60761-789-1_9 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;659:129-45. doi: 10.1007/978-1-60761-789-1_9.