PMID- 20809315
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20100902
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 659
DP  - 2010
TI  - Characterization of chromosomal rearrangements using multicolor-banding 
      (MCB/m-band).
PG  - 231-8
LID - 10.1007/978-1-60761-789-1_16 [doi]
AB  - Molecular cytogenetics and especially fluorescence in situ hybridization (FISH) 
      banding approaches are nowadays standard for the exact characterization of 
      simple, complex, and cryptic chromosomal aberrations within the human genome. 
      FISH-banding techniques are any kind of FISH techniques, which provide the 
      possibility to characterize simultaneously several chromosomal subregions smaller 
      than a chromosome arm. FISH banding methods fitting that definition may have 
      quite different characteristics, but share the ability to produce a DNA-specific 
      chromosomal banding. While the standard techniques such as G-bands by Trypsin 
      using Giemsa banding lead to a protein-related black and white banding pattern, 
      FISH-banding techniques are DNA-specific, more colorful, and thus, more 
      informative. At present, the most frequently applied FISH banding technique is 
      the multicolor banding (MCB/m-band) approach. MCB/m-band is based on 
      region-specific microdissection libraries, producing changing fluorescence 
      intensity ratios along the chromosomes. Here we describe the FISH-banding 
      technique MCB/m-band and illustrate how to apply it for characterization of 
      chromosomal breakpoints with a minimal number of FISH experiments.
FAU - Liehr, Thomas
AU  - Liehr T
AD  - Institut fur Humangenetik und Anthropologie, University of Jena, Jena, Germany. 
      i8lith@mti.uni-jena.de
FAU - Weise, Anja
AU  - Weise A
FAU - Hinreiner, Sophie
AU  - Hinreiner S
FAU - Mkrtchyan, Hasmik
AU  - Mkrtchyan H
FAU - Mrasek, Kristin
AU  - Mrasek K
FAU - Kosyakova, Nadezda
AU  - Kosyakova N
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (Fluorescent Dyes)
RN  - 9007-49-2 (DNA)
SB  - IM
MH  - Chromosomes/*genetics
MH  - Color
MH  - DNA/genetics/metabolism
MH  - Fluorescent Dyes/metabolism
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Denaturation
EDAT- 2010/09/03 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - 10.1007/978-1-60761-789-1_16 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;659:231-8. doi: 10.1007/978-1-60761-789-1_16.