PMID- 20809318
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20100902
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 659
DP  - 2010
TI  - FlashFISH: "same day" prenatal diagnosis of common chromosomal aneuploidies.
PG  - 261-8
LID - 10.1007/978-1-60761-789-1_19 [doi]
AB  - Fluorescence in situ hybridization (FISH) and quantitative fluorescence (QF)-PCR 
      are rapid molecular methods that test for common chromosomal aneuploidies in 
      prenatal diagnosis. While cytogenetic analysis requires approximately 7-14 days 
      before fetal karyotypes are available, these molecular methods release results of 
      sex chromosome aneuploidies, Down syndrome, Edward's syndrome, and Patau's 
      syndrome within 24-48 h of fetal sampling, alleviating parental anxiety. However, 
      specific diagnosis or exclusion of aneuploidy should be available within the same 
      day of amniocentesis. We developed "FlashFISH," a low cost FISH method that 
      allows accurate results to be reported within 2 h of fetal sampling. Here, we 
      report our experience of using FlashFISH in prenatal diagnosis, and we illustrate 
      in detail the protocols used for the purpose in our laboratory.
FAU - Ho, Sherry S Y
AU  - Ho SS
AD  - Department of Obstetrics and Gynaecology, Yong Loo Lin School of Medicine, 
      National University of Singapore, Singapore, Singapore.
FAU - Choolani, Mahesh A
AU  - Choolani MA
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Adult
MH  - Amniotic Fluid/cytology
MH  - *Aneuploidy
MH  - Chorionic Villi/pathology
MH  - Chromosomes/*genetics
MH  - Female
MH  - Humans
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Infant, Newborn
MH  - Pregnancy
MH  - Prenatal Diagnosis/*methods
MH  - Time Factors
EDAT- 2010/09/03 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - 10.1007/978-1-60761-789-1_19 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;659:261-8. doi: 10.1007/978-1-60761-789-1_19.