PMID- 20809323
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20100902
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 659
DP  - 2010
TI  - ImmunoFISH on isolated nuclei from paraffin-embedded biopsy material.
PG  - 313-21
LID - 10.1007/978-1-60761-789-1_24 [doi]
AB  - The detection of genetic abnormalities in paraffin sections by fluorescence in 
      situ hybridization (FISH) is widely used in clinical practice to detect 
      amplification of the ERB2 gene in breast carcinoma and various chromosomal 
      translocations in lymphomas and soft tissue tumors. However, interpretation of 
      FISH signals in tissue sections may be difficult due to overlapping nuclei and 
      nuclear truncation artifacts. Some of these shortcomings may be avoided by the 
      use of isolated nuclear preparations. However, identification of cell populations 
      may be difficult in detached cells removed from their histological context. We 
      have described an optimized immunoFISH technique on isolated nuclear suspension, 
      which combines the benefits of studying isolated cells derived from paraffin 
      embedded tissues by FISH analysis with the ability to detect cell lineage and 
      other markers by immunofluorescence.
FAU - Tan, Soo-Yong
AU  - Tan SY
AD  - Department of Pathology, Singapore General Hospital, Singapore, Singapore. 
      gpttsy@sgh.com.sg
FAU - Mattsson, Goran
AU  - Mattsson G
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Biopsy
MH  - Cell Nucleus/*metabolism
MH  - Cell Separation
MH  - Fluorescent Antibody Technique
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Paraffin Embedding/*methods
EDAT- 2010/09/03 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - 10.1007/978-1-60761-789-1_24 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;659:313-21. doi: 10.1007/978-1-60761-789-1_24.