PMID- 20809328
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20100902
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 659
DP  - 2010
TI  - FISH on chromosomes derived from the snail model organism Biomphalaria glabrata.
PG  - 379-88
LID - 10.1007/978-1-60761-789-1_29 [doi]
AB  - The application of fluorescence in situ hybridization (FISH) for the mapping of 
      single copy genes onto homologous chromosome has been integral to vast number 
      genome sequencing projects, such as that of mouse and human. The chromosomes of 
      these organisms are well-studied and are the staple resource of most of the early 
      studies conducted in cytogenetics. However, there are now protocols for analyzing 
      FISH probes in a number of different organisms on both metaphase and interphase 
      chromosomes.Here, we describe the methodologies for the chromosomal mapping of 
      nonrepetitive (single-copy) genes of the snail Biomphalaria glabrata onto 
      metaphase chromosomes derived from the only molluscan cell-line in existence. The 
      technique described in this chapter was developed for the B. glabrata genome 
      sequencing project through troubleshooting experimental procedures established 
      for other organisms so that both the optimum resolution of metaphase chromosome 
      and the effective hybridization of genes were achieved.
FAU - Odoemelam, Edwin C
AU  - Odoemelam EC
AD  - Division of Biosciences, Brunel University, Uxbridge, Middlesex, UK.
FAU - Raghavan, Nithya
AU  - Raghavan N
FAU - Ittiprasert, Wannaporn
AU  - Ittiprasert W
FAU - Miller, Andre
AU  - Miller A
FAU - Bridger, Joanna M
AU  - Bridger JM
FAU - Knight, Matty
AU  - Knight M
LA  - eng
GR  - R01-AI63480/AI/NIAID NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Biomphalaria/cytology/*genetics
MH  - Cell Culture Techniques
MH  - Chromosome Mapping/*methods
MH  - Genome/genetics
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Nucleic Acid Denaturation
EDAT- 2010/09/03 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - 10.1007/978-1-60761-789-1_29 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;659:379-88. doi: 10.1007/978-1-60761-789-1_29.