PMID- 20809331
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20100902
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 659
DP  - 2010
TI  - Fluorescence in situ hybridization (FISH) for genomic investigations in rat.
PG  - 409-26
LID - 10.1007/978-1-60761-789-1_32 [doi]
AB  - This chapter concentrates on the use of fluorescence in situ hybridization (FISH) 
      for genomic investigations in the laboratory rat (Rattus norvegicus). The 
      selection of protocols included in the chapter has been inspired by a 
      comprehensive range of previously published molecular cytogenetic studies on this 
      model organism, reporting examples of how FISH can be applied for diverse 
      investigative purposes, varying from comparative gene mapping to studies of 
      chromosome structure and genome evolution, to characterization of chromosomes 
      aberrations as well as transgenic insertions. The protocols, which include 
      techniques for the preparation of mitotic chromosomes and DNA fibers from 
      short-term cell cultures, have been gathered through the years and repeatedly 
      tested in our laboratory, and all together aim at providing sufficient 
      experimental versatility to cover a broad range of cytogenetic and cytogenomic 
      applications.
FAU - Jefferson, Andrew
AU  - Jefferson A
AD  - Department of Cardiovascular Medicine, John Radcliffe Hospital, Oxford, UK.
FAU - Volpi, Emanuela V
AU  - Volpi EV
LA  - eng
PT  - Journal Article
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
RN  - 0 (DNA Probes)
SB  - IM
MH  - Animals
MH  - Cell Culture Techniques
MH  - Cell Line
MH  - Cell Transformation, Viral
MH  - Chromosomes/metabolism
MH  - DNA Probes/genetics/metabolism
MH  - Fibroblasts/cytology/metabolism
MH  - Genomics/*methods
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Lymphocytes/cytology
MH  - Microscopy, Fluorescence
MH  - Rats
EDAT- 2010/09/03 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - 10.1007/978-1-60761-789-1_32 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;659:409-26. doi: 10.1007/978-1-60761-789-1_32.