PMID- 20809333
OWN - NLM
STAT- MEDLINE
DCOM- 20101214
LR  - 20100902
IS  - 1940-6029 (Electronic)
IS  - 1064-3745 (Linking)
VI  - 659
DP  - 2010
TI  - FISH on 3D preserved bovine and murine preimplantation embryos.
PG  - 437-45
LID - 10.1007/978-1-60761-789-1_34 [doi]
AB  - Fluorescence in situ hybridization (FISH) is a commonly used technique for the 
      visualization of whole chromosomes or subchromosomal regions, such as chromosome 
      arms, bands, centromeres, or single gene loci. FISH is routinely performed on 
      chromosome spreads, as well as on three-dimensionally preserved cells or tissues 
      (3D FISH). We have developed 3D FISH protocol for mammalian preimplantation 
      embryos to investigate the nuclear organization of chromosome territories and 
      subchromosomal regions during the first developmental stages. In contrast to 
      cells, embryos have much more depth and their nuclei are therefore less 
      accessible to probes used to visualize specific genomic regions by FISH. The 
      present protocol was developed to establish a balance between sufficient embryo 
      permeabilization and maximum preservation of nuclear morphology.
FAU - Koehler, Daniela
AU  - Koehler D
AD  - Institute of Human Genetics, Biozentrum (LMU), University of Munich, 
      Planegg-Martinsried, Germany. Dani.Koehler@lrz.uni-muenchen.de1
FAU - Zakhartchenko, Valeri
AU  - Zakhartchenko V
FAU - Ketterl, Nina
AU  - Ketterl N
FAU - Wolf, Eckhard
AU  - Wolf E
FAU - Cremer, Thomas
AU  - Cremer T
FAU - Brero, Alessandro
AU  - Brero A
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
PL  - United States
TA  - Methods Mol Biol
JT  - Methods in molecular biology (Clifton, N.J.)
JID - 9214969
SB  - IM
MH  - Animals
MH  - Blastocyst/*cytology/metabolism
MH  - Cattle
MH  - Imaging, Three-Dimensional
MH  - In Situ Hybridization, Fluorescence/*methods
MH  - Mice
MH  - Microscopy, Fluorescence
MH  - Nucleic Acid Denaturation
MH  - Tissue Embedding
MH  - Tissue Fixation
MH  - Tissue Preservation/*methods
EDAT- 2010/09/03 06:00
MHDA- 2010/12/16 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/16 06:00 [medline]
AID - 10.1007/978-1-60761-789-1_34 [doi]
PST - ppublish
SO  - Methods Mol Biol. 2010;659:437-45. doi: 10.1007/978-1-60761-789-1_34.