PMID- 20809695
OWN - NLM
STAT- MEDLINE
DCOM- 20110801
LR  - 20220409
IS  - 1557-8534 (Electronic)
IS  - 1547-3287 (Linking)
VI  - 20
IP  - 4
DP  - 2011 Apr
TI  - New strategies for anterior cruciate ligament partial rupture using bone marrow 
      transplantation in rats.
PG  - 671-9
LID - 10.1089/scd.2010.0182 [doi]
AB  - The purpose of this study was to compare anterior cruciate ligament (ACL) 
      regeneration between animal groups subjected to intra-articular injection of 
      fresh whole bone marrow cells (BMCs), cultured mesenchymal stem cells (MSCs), or 
      saline. Partially transected ACLs in Fischer 344/Nslc rats were prepared, 
      followed by injection of BMCs, MSCs, or saline into the articular cavity at 1 
      week after transection. Donor cells expressing green fluorescent protein were 
      detected in the recipient's transected ACLs at 4 weeks in the BMC and MSC groups, 
      and their ACLs appeared almost normal histologically. Further, there were 
      significantly more mature spindle cells in the BMC group than in the saline group 
      at 4 weeks. Biomechanically, the tensile strength in the BMC group reached near 
      normal levels at 4 weeks after injection. The levels of transforming growth 
      factor-beta1 in the ACL tissue and knee joint fluid in the BMC group were increased 
      significantly compared with that of the saline group at 4 weeks as detected by 
      immunohistochemical analysis. In conclusion, intra-articular bone marrow 
      transplantation using fresh whole BMCs is an effective treatment for ACL partial 
      rupture. This therapy is easy to apply in a clinical setting because no culture 
      system is required for collecting MSCs.
FAU - Oe, Kenichi
AU  - Oe K
AD  - Department of Orthopaedic Surgery, Kansai Medical University, Moriguchi City, 
      Osaka, Japan.
FAU - Kushida, Taketoshi
AU  - Kushida T
FAU - Okamoto, Naofumi
AU  - Okamoto N
FAU - Umeda, Masayuki
AU  - Umeda M
FAU - Nakamura, Tomohisa
AU  - Nakamura T
FAU - Ikehara, Susumu
AU  - Ikehara S
FAU - Iida, Hirokazu
AU  - Iida H
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20101023
PL  - United States
TA  - Stem Cells Dev
JT  - Stem cells and development
JID - 101197107
RN  - 0 (Collagen Type I)
RN  - 0 (Cytokines)
RN  - 0 (Transforming Growth Factor beta1)
SB  - IM
MH  - Animals
MH  - Anterior Cruciate Ligament/pathology/surgery
MH  - *Anterior Cruciate Ligament Injuries
MH  - Bone Marrow Transplantation/*methods
MH  - Cells, Cultured
MH  - Collagen Type I/metabolism
MH  - Cytokines/metabolism
MH  - Knee Joint/metabolism
MH  - Male
MH  - Mesenchymal Stem Cell Transplantation/*methods
MH  - Rats
MH  - Rats, Inbred F344
MH  - Rupture
MH  - Tensile Strength
MH  - Transcription, Genetic
MH  - Transforming Growth Factor beta1/genetics/metabolism
MH  - Up-Regulation
EDAT- 2010/09/03 06:00
MHDA- 2011/08/02 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2011/08/02 06:00 [medline]
AID - 10.1089/scd.2010.0182 [doi]
PST - ppublish
SO  - Stem Cells Dev. 2011 Apr;20(4):671-9. doi: 10.1089/scd.2010.0182. Epub 2010 Oct 
      23.