PMID- 20810175
OWN - NLM
STAT- MEDLINE
DCOM- 20101216
LR  - 20121115
IS  - 1879-1514 (Electronic)
IS  - 0166-445X (Linking)
VI  - 100
IP  - 3
DP  - 2010 Nov 1
TI  - 2,4,6-Trichlorophenol mediated increases in extracellular peroxidase activity in 
      three species of Lemnaceae.
PG  - 289-94
LID - 10.1016/j.aquatox.2010.08.001 [doi]
AB  - Chlorinated phenols, or chlorophenols, are persistent priority pollutants that 
      are widespread in the environment. Class III peroxidases are well-characterised 
      plant enzymes that can catalyse the oxidative dechlorination of chlorophenols. 
      Expression of these enzymes by plants is commonly associated with plant stress, 
      therefore limiting scope for phytoremediation. In this study, we have 
      quantitatively compared peroxidase activity and phytotoxicity as a function of 
      2,4,6-trichlorophenol (TCP) concentration in three species of Lemnaceae; Lemna 
      minor, Lemna gibba and Landoltia punctata. Effects of TCP on the growth rates of 
      the three species differed considerably with L. punctata being the most tolerant 
      species. TCP also affected photosynthetic parameters, causing a decrease in open 
      photosystem II reaction centres (qP) and, in L. punctata only, a decrease in 
      non-photochemical quenching (qN). In parallel, TCP exposure resulted in increased 
      peroxidase activity in all three species. Peroxidase activity in L. minor and L. 
      gibba displayed an inverse relationship with biomass accumulation, i.e. the more 
      growth reduction the more peroxidase activity. In contrast, induction of 
      peroxidase activity in L. punctata was bi-phasic, with a TCP-induced activity 
      peak at concentrations that had no major effect on growth, and further induction 
      under phytotoxic concentrations. The mechanism by which L. punctata recognises 
      and responds to low concentrations of an anthropogenic compound, in the absence 
      of wide-ranging stress, remains enigmatic. However, we conclude that this 
      "window" of peroxidase production in the absence of major growth inhibition 
      offers potential for the development of sustainable, peroxidise-mediated 
      phytoremediation systems.
CI  - Copyright (c) 2010 Elsevier B.V. All rights reserved.
FAU - Biswas, Dilip K
AU  - Biswas DK
AD  - Department of Zoology, Ecology and Plant Science, University College Cork, Cork, 
      Ireland.
FAU - Scannell, Gillian
AU  - Scannell G
FAU - Akhmetov, Nurlan
AU  - Akhmetov N
FAU - Fitzpatrick, Dara
AU  - Fitzpatrick D
FAU - Jansen, Marcel A K
AU  - Jansen MA
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20100812
PL  - Netherlands
TA  - Aquat Toxicol
JT  - Aquatic toxicology (Amsterdam, Netherlands)
JID - 8500246
RN  - 0 (Chlorophenols)
RN  - 0 (Water Pollutants, Chemical)
RN  - EC 1.11.1.7 (Peroxidase)
RN  - MHS8C5BAUZ (2,4,6-trichlorophenol)
SB  - IM
MH  - Araceae/*drug effects/enzymology/metabolism
MH  - Chlorophenols/*toxicity
MH  - Peroxidase/analysis/*metabolism
MH  - Photosynthesis/drug effects
MH  - Water Pollutants, Chemical/toxicity
EDAT- 2010/09/03 06:00
MHDA- 2010/12/17 06:00
CRDT- 2010/09/03 06:00
PHST- 2010/05/14 00:00 [received]
PHST- 2010/07/30 00:00 [revised]
PHST- 2010/08/05 00:00 [accepted]
PHST- 2010/09/03 06:00 [entrez]
PHST- 2010/09/03 06:00 [pubmed]
PHST- 2010/12/17 06:00 [medline]
AID - S0166-445X(10)00301-2 [pii]
AID - 10.1016/j.aquatox.2010.08.001 [doi]
PST - ppublish
SO  - Aquat Toxicol. 2010 Nov 1;100(3):289-94. doi: 10.1016/j.aquatox.2010.08.001. Epub 
      2010 Aug 12.